Evidence map›Paper›PMID 32276040›Full record

ArticleBiomaterials2020

Decoupling the effects of nanopore size and surface roughness on the attachment, spreading and differentiation of bone marrow-derived stem cells.

Jing Xia, Yuan Yuan, Huayin Wu, Yuting Huang, David A Weitz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jing XiaSchool of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA.
Yuan YuanSchool of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA.
Huayin WuSchool of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA.
Yuting HuangSchool of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA.
David A WeitzSchool of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA; Department of Physics, Harvard University, Cambridge, MA, 02138, USA. Electronic address: weitz@seas.harvard.edu.

Funding

MSC Encapsulation with Thin Gel CoatingR01EB023287 · NIBIB · HARVARD UNIVERSITY · PI MOONEY, DAVID J, SCADDEN, DAVID T · 2017 to 2019
$2.0M
NIBIB NIH HHS R01 EB023287
6 · The paper itself

Abstract

The nanopore size and roughness of nanoporous surface are two critical variables in determining stem cell fate, but little is known about the contribution from each cue individually. To address this gap, we use two-dimensional nanoporous membranes with controlled nanopore size and roughness to culture bone marrow-derived mesenchymal stem cells (BMSCs), and study their behaviors such as attachment, spreading and differentiation. We find that increasing the roughness of nanoporous surface has no noticeable effect on cell attachment, and only slightly decreases cell spreading areas and inhibits osteogenic differentiation. However, BMSCs cultured on membranes with larger nanopores have significantly fewer attached cells and larger spreading areas. Moreover, these cells cultured on larger nanopores undergo enhanced osteogenic differentiation by expressing more alkaline phosphatase, osteocalcin, osteopontin, and secreting more collagen type I. These results suggest that although both nanopore size and roughness can affect BMSCs, nanopore size plays a more significant role than roughness in controlling BMSC behavior.

Indexed as

NanoporesOsteogenesisBone MarrowBone Marrow CellsCell DifferentiationCells, CulturedCell morphologyMesenchymal stem cellNanopore sizeOsteogenic differentiationRoughnessTwo-dimensional nanoporous surface

Identifiers

PMID32276040
PMCPMC7262959

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.