Evidence map›Paper›PMID 32274442›Full record

ArticleAmerican journal of ophthalmology case reports2020

Proteomic analysis of intermediate uveitis suggests myeloid cell recruitment and implicates IL-23 as a therapeutic target.

Yasir J Sepah, Gabriel Velez, Peter H Tang, Jing Yang, Teja Chemudupati, Angela S Li, Quan D Nguyen, Alexander G Bassuk, Vinit B Mahajan

Abstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of ophthalmology case reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Serum Biomarkers of Vascular Involvement in Childhood Uveitis.Translational vision science & technology · 2024
    Article
  6. Liquid Biopsy Proteomics in Ophthalmology.Journal of proteome research · 2024
    Review
  7. Is tear proteome profile a predictor of developing uveitis in ANA-positive patients with oligoarticular juvenile idiopathic arthritis?Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2024
    Article
  8. Review
  9. Intraoperative Complications With Vitreous Biopsy for Molecular Proteomics.Ophthalmic surgery, lasers & imaging retina · 2023
    Article
  10. Review
  11. Review
  12. Review
  13. Protein Biomarkers in Uveitis.Frontiers in immunology · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yasir J SepahOmics Laboratory, Stanford University, Palo Alto, CA, USA.
Gabriel VelezOmics Laboratory, Stanford University, Palo Alto, CA, USA.
Peter H TangOmics Laboratory, Stanford University, Palo Alto, CA, USA.
Jing YangOmics Laboratory, Stanford University, Palo Alto, CA, USA.
Teja ChemudupatiOmics Laboratory, Stanford University, Palo Alto, CA, USA.
Angela S LiOmics Laboratory, Stanford University, Palo Alto, CA, USA.
Quan D NguyenDepartment of Ophthalmology, Byers Eye Institute, Stanford University, Palo Alto, CA, USA.
Alexander G BassukDepartment of Pediatrics, University of Iowa, Iowa City, IA, USA.
Vinit B MahajanOmics Laboratory, Stanford University, Palo Alto, CA, USA.

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM007337 · NIGMS · UNIVERSITY OF IOWA · PI GEYER, PAMELA K., LENTZ, STEVEN R · 1985 to 2020
$15.9M
NIGMS NIH HHS T32 GM007337
6 · The paper itself

Abstract

purposeTo profile vitreous protein expression of intermediate uveitis (IU) patients. OBSERVATIONS: We identified a mean of 363 ± 41 unique proteins (mean ± SD) in IU vitreous and 393 ± 69 unique proteins in control samples using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of liquid vitreous biopsies collected during pars plana vitrectomy. A total of 233 proteins were differentially expressed among control and IU samples, suggesting a protein signature that could distinguish the two groups. Pathway analysis identified 22 inflammatory mediators of the interleukin-12 (IL-12) signaling pathway in IU vitreous. Upstream regulator analysis identified downstream mediators of IL-23 and myeloid differentiation primary response protein (MYD88), both of which are involved in the recruitment and differentiation of myeloid cells. Taken together, our results suggest the recruitment of myeloid cells as an upstream pathway in the pathogenesis of IU.

conclusionsThis study provides insights into proteins that will serve as biomarkers and therapeutic targets for IU. These biomarkers will help design future clinical trials using rational molecular therapeutics.

Indexed as

BiomarkerIntermediate uveitisProteomicsVitreous

Identifiers

PMID32274442
PMCPMC7132169

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.