Evidence map›Paper›PMID 32273819›Full record

ArticleReproductive medicine and biology2020

Hollow fiber vitrification allows cryopreservation of embryos with compromised cryotolerance.

Ayuko Uchikura, Hitomi Matsunari, Miki Maehara, Shiori Yonamine, Sayaka Wakayama, Teruhiko Wakayama, Hiroshi Nagashima

Open access · goldAbstract read
In one paragraph

Article in Reproductive medicine and biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Ayuko UchikuraLaboratory of Developmental Engineering Department of Life Sciences School of Agriculture Meiji University Kawasaki Japan.ORCID https://orcid.org/0000-0002-1535-3808
Hitomi MatsunariLaboratory of Developmental Engineering Department of Life Sciences School of Agriculture Meiji University Kawasaki Japan.
Miki MaeharaDepartment of Orthopaedic Surgery Surgical Science Tokai University School of Medicine Isehara Japan.
Shiori YonamineLaboratory of Developmental Engineering Department of Life Sciences School of Agriculture Meiji University Kawasaki Japan.
Sayaka WakayamaDepartment of Biotechnology Faculty of Life and Environmental Sciences University of Yamanashi Kohu Japan.
Teruhiko WakayamaDepartment of Biotechnology Faculty of Life and Environmental Sciences University of Yamanashi Kohu Japan.
Hiroshi NagashimaLaboratory of Developmental Engineering Department of Life Sciences School of Agriculture Meiji University Kawasaki Japan.
Meiji University · JPUniversity of Yamanashi · JPTokai University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThis study aims to demonstrate vitrification methods that provide reliable cryopreservation for embryos with compromised cryotolerance.

methodsTwo-cell stage mouse embryos and in vitro produced porcine embryos were vitrified using the hollow fiber vitrification (HFV) and Cryotop (CT) methods. The performance of these two methods was compared by the viability of the vitrified-rewarmed embryos.

resultsRegardless of the method used, 100% of the mouse 2-cell embryos developed successfully after vitrification-rewarming into the blastocyst stage, whereas vitrification tests using porcine morulae with the HFV method produced significantly better results. The developmental rates of vitrified porcine morula into the blastocyst stage, as well as blastocyst cell number, were 90.3% and 112.3 ± 6.9 in the HFV group compared with 63.4% and 89.5 ± 8.1 in the CT group (

conclusionVitrification test using embryos with compromised cryotolerance allows for more precise determining of effective cryopreservation methods and devices.

Indexed as

cryotoleranceembryo cryopreservationhollow fiber vitrification (HFV)mouse embryosporcine embryos

Identifiers

PMID32273819
PMCPMC7138943
OpenAlexW2995127303

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.