ArticleScience (New York, N.Y.)2020
Intravital imaging of mouse embryos.
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Article in Science (New York, N.Y.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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58citing papers in PubMed
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Who cites it
58 citing papers in PubMed.
- Agrin-Enriched Neonatal Spinal Cord-Derived Extracellular Vesicles Bypass Age Barriers to Organize Spinal Cord Repair.Journal of extracellular vesicles · 2026Article
- Endothelial Continuum and Capillary Specialization in Pulmonary Vascular Development.Arteriosclerosis, thrombosis, and vascular biology · 2026Review
- Para-uterine imaging protocol: Stabilizing living mouse embryos within the maternal abdomen for in vivo imaging and visually guided physiology.STAR protocols · 2026Article
- Contrast-agent-free visualization of murine embryo-placental vasculature with ultrafast plane-wave ultrasound.Placenta · 2026Article
- Longitudinal in vivo OCT/OCTA of gastric tumor microvasculature via an implantable optical window.Biomedical optics express · 2026Article
- Time-of-day difference in iron supplementation in iron-deficient pregnant model mice.NPJ science of food · 2026Article
- Sound of nurturing: Advanced photoacoustic and ultrasound imaging of placental hemodynamics.Placenta · 2026Article
- Rapid 3D Immunolabeling and Light Sheet Microscopy for Quantitative Analysis of Intact Tissues.Computational and structural biotechnology journal · 2026Article
- Review
- Embryoid bodies as a model system for exploring early human embryonic development.Journal of assisted reproduction and genetics · 2025Review
- Accessing the viscera: Technologies for interoception research.Current opinion in neurobiology · 2025Review
- Deep-tissue two-photon brain imaging enabled by a tunable fiber-optic dispersive wave generator.Scientific reports · 2025Article
- Article
- Long-term intravital subcellular imaging with confocal scanning light-field microscopy.Nature biotechnology · 2025Article
- 4-Phenyl Butyric Acid (4-PBA) Suppresses Neutrophil Recruitment in a Murine Model of Acute Perinatal Inflammation.Journal of immunology research · 2025Article
- In vivo imaging of vagal-induced myenteric plexus responses in gastrointestinal tract with an optical window.Nature communications · 2024Article
- Network state transitions during cortical development.Nature reviews. Neuroscience · 2024Review
- In Vivo Imaging of Rabbit Follicles through Combining Ultrasound Bio-Microscopy and Intravital Window.Animals : an open access journal from MDPI · 2024Article
- Decoding the universal human chromatin landscape through teratoma-based profiling.Nucleic acids research · 2024Article
- Longitudinal intravital imaging of mouse placenta.Science advances · 2024Article
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Authors and funding
27 authors.
Qiang HuangDepartment of Pediatric Surgery, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. drhuang@xjtu.edu.cn jaenisch@wi.mit.edu xs37@duke.edu.ORCID 0000-0001-9040-4591
Malkiel A CohenWhitehead Institute for Biomedical Research, Cambridge, MA, USA.ORCID 0000-0002-4187-691X
Fernando C AlsinaDepartment of Molecular Genetics and Microbiology, School of Medicine, Duke University, Durham, NC, USA.ORCID 0000-0002-6304-7688
Garth DevlinDepartment of Surgery, School of Medicine, Duke University, Durham, NC, USA.
Aliesha GarrettDepartment of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.ORCID 0000-0002-6618-3036
Jennifer McKeyDepartment of Cell Biology, School of Medicine, Duke University, Durham, NC, USA.ORCID 0000-0002-2640-1502
Patrick HavlikDepartment of Surgery, School of Medicine, Duke University, Durham, NC, USA.
Nikolai RakhilinDepartment of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.
Ergang WangDepartment of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.ORCID 0000-0003-2550-3686
Kun XiangDepartment of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.ORCID 0000-0001-7793-4352
Parker MathewsDivision of Hematologic Malignancies and Cellular Therapy, Duke University Medical Center, Durham, NC, USA.
Lihua WangDepartment of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.
Cheryl BockDuke Cancer Institute, School of Medicine, Duke University, Durham, NC, USA.
Victor RuthigDepartment of Cell Biology, School of Medicine, Duke University, Durham, NC, USA.ORCID 0000-0003-1126-6106
Yi WangDepartment of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.ORCID 0000-0001-7988-2939
Marcos NegreteDepartment of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.ORCID 0000-0002-1169-2159
Chi Wut WongDepartment of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.ORCID 0000-0002-1703-785X
Preetish K L MurthyDepartment of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA.ORCID 0000-0002-9762-8376
Shupei ZhangWhitehead Institute for Biomedical Research, Cambridge, MA, USA.
Andrea R DanielDepartment of Radiation Oncology, Duke University Medical Center, Durham, NC, USA.ORCID 0000-0003-2186-5911
David G KirschDepartment of Radiation Oncology, Duke University Medical Center, Durham, NC, USA.ORCID 0000-0002-2086-205X
Yubin KangDivision of Hematologic Malignancies and Cellular Therapy, Duke University Medical Center, Durham, NC, USA.ORCID 0000-0001-6678-8732
Blanche CapelDepartment of Cell Biology, School of Medicine, Duke University, Durham, NC, USA.ORCID 0000-0002-6587-0969
Aravind AsokanDepartment of Surgery, School of Medicine, Duke University, Durham, NC, USA.ORCID 0000-0001-5563-4877
Debra L SilverDepartment of Molecular Genetics and Microbiology, School of Medicine, Duke University, Durham, NC, USA.ORCID 0000-0001-9189-844X
Rudolf JaenischWhitehead Institute for Biomedical Research, Cambridge, MA, USA. drhuang@xjtu.edu.cn jaenisch@wi.mit.edu xs37@duke.edu.ORCID 0000-0002-2540-7099
Xiling ShenDepartment of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, NC, USA. drhuang@xjtu.edu.cn jaenisch@wi.mit.edu xs37@duke.edu.ORCID 0000-0002-4978-3531
Funding
Advancing Cancer Therapy through Ground Breaking Research in Radiation BiologyR35CA197616 · NCI · ROSWELL PARK CANCER INSTITUTE CORP · PI David Guy Kirsch · 2016 to 2026
$10.7MIn Vitro Reprogramming of Somatic Cells into Pluripotent ES-like CellsR37HD045022 · NICHD · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI JAENISCH, RUDOLF · 2009 to 2017
$7.5MTranscriptional condensates, epigenetic editing and Rett SyndromeR01MH104610 · NIMH · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI JAENISCH, RUDOLF, YOUNG, RICHARD · 2014 to 2023
$7.4MRobust Control of the Stem Cell NicheR35GM122465 · NIGMS · TERASAKI INSTITUTE FOR BIOMEDICAL INNOVATION · PI Xiling Shen · 2017 to 2026
$5.6MAn iPSC based platform for functionally assessing genetic and environmental riskR01NS088538 · NINDS · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI JAENISCH, RUDOLF, MYERS, RICHARD H · 2014 to 2018
$5.0MOPPOSING PATHWAYS IN MAMMALIAN SEX DETERMINATIONR01HD039963 · NICHD · DUKE UNIVERSITY · PI CAPEL, BLANCHE · 2001 to 2025
$4.7MMechanisms of neural progenitor division in the developing brainR01NS083897 · NINDS · DUKE UNIVERSITY · PI SILVER, DEBRA · 2013 to 2023
$3.4MAn organotypic model recapitulating colon cancer microenvironment and metastasisU01CA214300 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI HUANG, EMINA HUI-NA, SHEN, XILING · 2017 to 2021
$3.1MDeveloping a comprehensive model for peripheral nerve stimulation of gastrointestinal functionR01DK119795 · NIDDK · TERASAKI INSTITUTE FOR BIOMEDICAL INNOVATION · PI SHEN, XILING · 2019 to 2022
$1.8MDistal mRNA localization and translation in neural stem cells of the developing brainR01NS110388 · NINDS · DUKE UNIVERSITY · PI SILVER, DEBRA · 2018 to 2022
$1.8MPost-transcriptional RNA regulation in mammalian neural stem cellsR21NS098176 · NINDS · DUKE UNIVERSITY · PI SILVER, DEBRA · 2017 to 2018
$437kEpitranscriptomic Control of Local Gene Expression in Neural Stem CellsR21MH119813 · NIMH · DUKE UNIVERSITY · PI MEYER, KATHRYN D, SILVER, DEBRA · 2019 to 2020
$435kNCI NIH HHS R35 CA197616NCI NIH HHS U01 CA214300NICHD NIH HHS R01 HD039963NICHD NIH HHS R37 HD045022NIDDK NIH HHS R01 DK119795NIGMS NIH HHS R35 GM122465NIMH NIH HHS R01 MH104610NIMH NIH HHS R21 MH119813NINDS NIH HHS R01 NS083897NINDS NIH HHS R01 NS088538NINDS NIH HHS R01 NS110388NINDS NIH HHS R21 NS098176
Abstract
Embryonic development is a complex process that is unamenable to direct observation. In this study, we implanted a window to the mouse uterus to visualize the developing embryo from embryonic day 9.5 to birth. This removable intravital window allowed manipulation and high-resolution imaging. In live mouse embryos, we observed transient neurotransmission and early vascularization of neural crest cell (NCC)-derived perivascular cells in the brain, autophagy in the retina, viral gene delivery, and chemical diffusion through the placenta. We combined the imaging window with in utero electroporation to label and track cell division and movement within embryos and observed that clusters of mouse NCC-derived cells expanded in interspecies chimeras, whereas adjacent human donor NCC-derived cells shrank. This technique can be combined with various tissue manipulation and microscopy methods to study the processes of development at unprecedented spatiotemporal resolution.
Indexed as
Embryonic DevelopmentNeural CrestAnimalsBrainCell DivisionCell MovementChimeraElectroporationEmbryo, MammalianFemaleGene Transfer TechniquesIntravital MicroscopyMiceMice, TransgenicNeovascularization, PhysiologicPlacenta
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