Evidence map›Paper›PMID 32272965›Full record

ArticleArthritis research & therapy2020

Bone marrow mesenchymal stem cell-derived exosomal microRNA-124-3p attenuates neurological damage in spinal cord ischemia-reperfusion injury by downregulating Ern1 and promoting M2 macrophage polarization.

Ran Li, Kunchi Zhao, Qing Ruan, Chunyang Meng, Fei Yin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arthritis research & therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
99citing papers in PubMed, 3 pooled it
7.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

99 citing papers in PubMed, 3 syntheses or guidelines pooled it, 150 citations in OpenAlex.

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39 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Ran LiDepartment of Spine Surgery, China-Japan Union Hospital, Jilin University, No. 126, Xiantai Street, Changchun, 130033, Jilin Province, People's Republic of China.
Kunchi ZhaoDepartment of Spine Surgery, China-Japan Union Hospital, Jilin University, No. 126, Xiantai Street, Changchun, 130033, Jilin Province, People's Republic of China. Kunchi2033@163.com.
Qing RuanDepartment of Spine Surgery, China-Japan Union Hospital, Jilin University, No. 126, Xiantai Street, Changchun, 130033, Jilin Province, People's Republic of China.
Chunyang MengDepartment of Spine Surgery, China-Japan Union Hospital, Jilin University, No. 126, Xiantai Street, Changchun, 130033, Jilin Province, People's Republic of China.
Fei YinDepartment of Spine Surgery, China-Japan Union Hospital, Jilin University, No. 126, Xiantai Street, Changchun, 130033, Jilin Province, People's Republic of China. yinfei999@jlu.edu.cn.
Jilin University · CNUnion Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSpinal cord ischemia-reperfusion injury (SCIRI) often leads to neurological damage and mortality. In this regard, understanding the pathology of SCIRI and preventing its development are of great clinic value.

methodsHerein, we analyzed the role of bone marrow mesenchymal stem cell (BMMSC)-derived exosomal microRNA (miR)-124-3p in SCIRI. A SCIRI rat model was established, and the expression of Ern1 and M2 macrophage polarization markers (Arg1, Ym1, and Fizz) was determined using immunohistochemistry, immunofluorescence assay, RT-qPCR, and western blot analysis. Targeting relationship between miR-124-3p and Ern1 was predicted using bioinformatic analysis and verified by dual-luciferase reporter assay. Macrophages were co-cultured with miR-124-3p-containing BMMSC-derived exosomes. M2 macrophages were identified using flow cytometry, and the expression of Arg1, Ym1, and Fizz was determined. In addition, SCIRI rats were injected with miR-124-3p-containing exosomes, spinal cord cell apoptosis was observed using TUNEL assay, and the pathological condition was evaluated with H&E staining.

resultsIn SCIRI, Ern1 was highly expressed and M2 polarization markers were poorly expressed. Silencing Ern1 led to elevated expression of M2 polarization markers. MiR-124-3p targeted and negatively regulated Ern1. Exosomal miR-124-3p enhanced M2 polarization. Highly expressed exosomal miR-124-3p impeded cell apoptosis and attenuated SCIRI-induced tissue impairment and nerve injury. miR-124-3p from BMMSC-derived exosomes ameliorated SCIRI and its associated nerve injury through inhibiting Ern1 and promoting M2 polarization.

conclusionIn summary, exosomal miR-124-3p derived from BMMSCs attenuated nerve injury induced by SCIRI by regulating Ern1 and M2 macrophage polarization.

Indexed as

AnimalsBone Marrow CellsCell DifferentiationDown-RegulationEndoribonucleasesExosomesGene Expression RegulationMacrophage ActivationMacrophagesMaleMesenchymal Stem CellsMicroRNAsMultienzyme ComplexesProtein Serine-Threonine KinasesRatsRats, Sprague-DawleyEndoribonucleasesErn1 protein, ratMicroRNAsMIRN124 microRNA, ratMultienzyme ComplexesProtein Serine-Threonine KinasesErn1ExosomeMacrophage M2MicroRNA-124-3pSpinal cord ischemia-reperfusion injury

Identifiers

PMID32272965
PMCPMC7146970
OpenAlexW3015674669

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.