Determining the Cytotoxicity of Oxidized Lipids in Cultured Caco-2 Cells Using Bioimaging Techniques.

Rabia Alghazeer, Abdullah A Burwaiss, Nazlin K Howell, Wafa S Alansari, Ghalia Shamlan, Areej A Eskandrani

Open access · goldAbstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.5field-weighted citation impact, top 23% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

Probe Cytotoxic and Oxidative Stress Effects of Nanoplastics on Caco-2 Cells: Insights from Raman Spectroscopy and Machine Learning.Sensors (Basel, Switzerland) · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 5 institutions in 3 countries.

Rabia AlghazeerChemistry Department, Faculty of Science, University of Tripoli, Tripoli 50676, Libya.

Abdullah A BurwaissMedicine Department, Faculty of Human Medicine, University of Tripoli, Tripoli 50676, Libya.

Nazlin K HowellSchool of Biomedical and Molecular Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK.

Wafa S AlansariBiochemistry Department, Faculty of Science, University of Jeddah, Jeddah 21577, Saudi Arabia.

Ghalia ShamlanDepartment of Food Science and Nutrition, College of Food and agriculture Sciences, King Saud University, Riyadh 11362, Saudi Arabia.

Areej A EskandraniChemistry Department, Faculty of Science, Taibah University, Medina 30002, Saudi Arabia.

University of Tripoli · LYKing Saud University · SATaibah University · SAUniversity of Jeddah · SAUniversity of Surrey · GB

Funding

Government of Libya 3000£

6 · The paper itself

Abstract

Fish lipids are comprised of considerable quantities of polyunsaturated acids and are prone to oxidation, producing reactive oxygen species and hydroperoxides. This study aimed to evaluate the biochemical and structural alterations in Caco-2 cells following exposure to 100 μg/mL methyl linoleate or fish oil, and then radiated for 24, 48 or 72 h. Electron spin resonance spectroscopy detected free radicals in the lipid membrane, Raman microscopy observed biochemical alterations and atomic force microscopy identified changes in morphology, such as the breakdown of DNA bonds. The study showed that bioimaging and biochemical techniques can be effective at detecting and diagnosing cellular injuries incurred by lipid peroxidation.

Indexed as

Caco-2 CellsCell Line, TumorElectron Spin Resonance SpectroscopyFish OilsFree RadicalsHumansHydrogen PeroxideLinoleic AcidLinoleic AcidsLipid PeroxidationMembrane LipidsOxidation-ReductionReactive Oxygen SpeciesFish OilsFree RadicalsHydrogen PeroxideLinoleic AcidLinoleic AcidsMembrane Lipidsmethyl linoleateReactive Oxygen SpeciesAFMESR spectroscopyfish oillipid peroxidationRaman microscopy

Identifiers

PMID32272768

DOI10.3390/molecules25071693

PMCPMC7180719

OpenAlexW3015459997

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.