ArticleMicroorganisms2020
Improved Protocol for DNA Extraction from Subsoils Using Phosphate Lysis Buffer.
Article in Microorganisms, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 60 citations in OpenAlex.
- Optimized Method for Efficient DNA Extraction from Agricultural Soils.Methods and protocols · 2026Article
- Optimising RNA extraction for paddy bulk soil samples for metatranscriptome sequencing.BMC research notes · 2025Article
- Development of Multiple Real-Time Fluorescent Quantitative PCR forVeterinary sciences · 2025Article
- Trees shape the soil microbiome of a temperate agrosilvopastoral and syntropic agroforestry system.Scientific reports · 2025Article
- Evaluation of oat genotypes for species-specific and cross-resistance toFrontiers in plant science · 2025Article
- Profile of Bacterial Communities in Copper Mine Tailings Revealed through High-Throughput Sequencing.Microorganisms · 2024Article
- Improving the efficiency of DNA extraction from iron incrustations and oilfield-produced water.Scientific reports · 2024Article
- An All-in-One Platform for On-Site Multiplex Foodborne Pathogen Detection Based on Channel-Digital Hybrid Microfluidics.Biosensors · 2024Article
- Development of a real-time recombinase-aided amplification assay for rapid and sensitive detection ofFrontiers in cellular and infection microbiology · 2024Article
- Synthetic Microbial Surrogates Consisting of Lipid Nanoparticles Encapsulating DNA for the Validation of Surface Disinfection Procedures.ACS applied bio materials · 2023Article
- Unique Habitat of Karst Tiankengs Changes the Taxonomy and Potential Metabolism of Soil Microbial Communities.Microbiology spectrum · 2023Article
- RIAM: A Universal Accessible Protocol for the Isolation of High Purity DNA from Various Soils and Other Humic Substances.Methods and protocols · 2022Article
- The Requirement of Genetic Diagnostic Technologies for Environmental Surveillance of Antimicrobial Resistance.Sensors (Basel, Switzerland) · 2021Article
- Relative Abundances of Species or Sequence Variants Can Be Misleading: Soil Fungal Communities as an Example.Microorganisms · 2021Article
- Tree rows in temperate agroforestry croplands alter the composition of soil bacterial communities.PloS one · 2021Article
- Article
- Development of EST-SSR Markers Linked to Flowering Candidate Genes inPlants (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
As our understanding of soil biology deepens, there is a growing demand for investigations addressing microbial processes in the earth beneath the topsoil layer, called subsoil. High clay content in subsoils often hinders the recovery of sufficient quantities of DNA as clay particles bind nucleic acids. Here, an efficient and reproducible DNA extraction method for 200 mg dried soil based on sodium dodecyl sulfate (SDS) lysis in the presence of phosphate buffer has been developed. The extraction protocol was optimized by quantifying bacterial 16S and fungal 18S rRNA genes amplified from extracts obtained by different combinations of lysis methods and phosphate buffer washes. The combination of one minute of bead beating, followed by ten min incubation at 65°C in the presence of 1 M phosphate buffer with 0.5% SDS, was found to produce the best results. The optimized protocol was compared with a commonly used cetyltrimethylammonium bromide (CTAB) method, using Phaeozem soil collected from 60 cm depth at a conventional agricultural field and validated on five subsoils. The reproducibility and robustness of the protocol was corroborated by an interlaboratory comparison. The DNA extraction protocol offers a reproducible and cost-effective tool for DNA-based studies of subsoil biology.
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Registered trials
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