ArticleMolecular metabolism2020
Skeletal muscle in healthy humans exhibits a day-night rhythm in lipid metabolism.
Article in Molecular metabolism, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- The effect of morning vs evening exercise training on glycaemic control and serum metabolites in overweight/obese men: a randomised trial.Diabetologia · 2021Trial
- Metabolic and muscular adaptations to dietary succinate in Tan sheep: Insights from transcriptomics.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Restoration of circadian rhythm as novel targets against sarcopenia.Chinese medical journal · 2026Review
- Nutritional adaptation to circadian misalignment: implications for musculoskeletal health in modern lifestyles.Frontiers in nutrition · 2026Review
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- Physiological rhythms and metabolic regulation: Shining light on skeletal muscle.Experimental physiology · 2025Review
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- Characterizing 24-Hour Skeletal Muscle Gene Expression Alongside Metabolic and Endocrine Responses Under Diurnal Conditions.The Journal of clinical endocrinology and metabolism · 2025Article
- Article
- A conserved complex lipid signature marks human muscle aging and responds to short-term exercise.Nature aging · 2024Article
- Circadian organization of lipid landscape is perturbed in type 2 diabetic patients.Cell reports. Medicine · 2023Article
- The Effect of Night Shifts on 24-h Rhythms in the Urinary Metabolome of Police Officers on a Rotating Work Schedule.Journal of biological rhythms · 2023Article
- Type 2 diabetes disrupts circadian orchestration of lipid metabolism and membrane fluidity in human pancreatic islets.PLoS biology · 2022Article
- In vitro characterisation of murine pre-adipose nucleated cells reveals electrophysiological cycles associated with biological clocks.Electrophoresis · 2022Article
- Nutrient timing and metabolic regulation.The Journal of physiology · 2022Review
- Untargeted and Targeted Circadian Metabolomics Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) and Flow Injection-Electrospray Ionization-Tandem Mass Spectrometry (FIA-ESI-MS/MS).Methods in molecular biology (Clifton, N.J.) · 2022Article
- Intrauterine Growth Restriction Induces Adulthood Chronic Metabolic Disorder in Cardiac and Skeletal Muscles.Frontiers in nutrition · 2022Article
- Circadian misalignment disturbs the skeletal muscle lipidome in healthy young men.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Tick-Tock Consider the Clock: The Influence of Circadian and External Cycles on Time of Day Variation in the Human Metabolome-A Review.Metabolites · 2021Review
- Metabolomics and lipidomics in Caenorhabditis elegans using a single-sample preparation.Disease models & mechanisms · 2021Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveHuman energy metabolism is under the regulation of the molecular circadian clock; we recently reported that mitochondrial respiration displays a day-night rhythm under study conditions that are similar to real life. Mitochondria are interconnected with lipid droplets, which are of importance in fuel utilization and play a role in muscle insulin sensitivity. Here, we investigated if skeletal muscle lipid content and composition also display day-night rhythmicity in healthy, lean volunteers.
methodsSkeletal muscle biopsies were obtained from 12 healthy lean male volunteers every 5 h over a 24 h period. Volunteers were provided with standardized meals, and biopsies were taken 4.5 h after each last meal. Lipid droplet size and number were investigated by confocal microscopy. Additionally, the muscle lipidome was assessed using UPLC/HRMS-based semi-targeted lipidomics.
resultsConfocal microscopy revealed diurnal differences in intramyocellular lipid content (P < 0.05) and lipid droplet size in oxidative type 1 muscle fibers (P < 0.01). Lipidomics analysis revealed that 13% of all detected lipids displayed significant day-night rhythmicity. The most rhythmic lipid species were glycerophospholipids and diacylglycerols (DAG), with the latter being the largest fraction (>50% of all rhythmic species). DAG levels showed a day-night pattern with a trough at 1 PM and a peak at 4 AM.
conclusionsUsing two distinct methods, our findings show that myocellular lipid content and whole muscle lipid composition vary across the day-night cycle under normal living conditions. In particular, day-night rhythmicity was present in over half of the DAG lipid species. Future studies are needed to investigate whether rhythmicity in DAG is functionally related to insulin sensitivity and how this might be altered in prediabetes.
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