ArticleScience advances2020
Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells.
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Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38citing papers in PubMed
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38 citing papers in PubMed, 69 citations in OpenAlex.
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Authors and funding
44 authors at 12 institutions in 7 countries.
Stephen D CarterDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.ORCID 0000-0002-4237-4276
Cheri M HamptonDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.ORCID 0000-0003-0069-8712
Robert LangloisDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.ORCID 0000-0003-1779-4786
Roberto MeleroBiocomputing Unit, Centro Nacional de Biotecnología-CSIC, Darwin 3, Campus Universidad Autónoma, 28049 Madrid, Spain.ORCID 0000-0001-9467-9381
Zachary J FarinoDepartment of Psychiatry, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Michael J CalderonDepartment of Cell Biology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Wen LiDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Callen T WallaceDepartment of Cell Biology, University of Pittsburgh, Pittsburgh, PA 15213, USA.ORCID 0000-0001-6852-4976
Ngoc Han TranHHMI, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA.ORCID 0000-0003-0638-2583
Robert A GrassucciDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.ORCID 0000-0002-1437-1537
Stephanie E SiegmundDepartment of Cellular, Molecular and Biophysical Studies, Columbia University Medical Center, New York, NY 10032, USA.ORCID 0000-0003-1848-2389
Joshua PembertonSection on Molecular Signal Transduction, Program for Developmental Neuroscience, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.ORCID 0000-0002-1492-1471
Travis J MorgensternDepartment of Psychiatry, Columbia University, New York, NY 10032, USA.ORCID 0000-0003-2634-8470
Leanna EisenmanDepartment of Neurobiology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Jenny I AguilarDepartment of Psychiatry, Columbia University, New York, NY 10032, USA.ORCID 0000-0001-7533-5153
Nili L GreenbergDepartment of Psychiatry, Columbia University, New York, NY 10032, USA.ORCID 0000-0002-8444-3487
Elana S LevyDepartment of Psychiatry, Columbia University, New York, NY 10032, USA.
Edward YiDepartment of Psychiatry, Columbia University, New York, NY 10032, USA.
William G MitchellDepartment of Psychiatry, Columbia University, New York, NY 10032, USA.ORCID 0000-0001-6030-520X
William J RiceNew York Structural Biology Center, New York, NY 10027, USA.ORCID 0000-0002-5187-255X
Christoph WiggeNew York Structural Biology Center, New York, NY 10027, USA.
Jyotsna PilliDepartment of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA 15261, USA.ORCID 0000-0002-6623-0883
Emily W GeorgeDepartment of Psychiatry, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Despoina AslanoglouDepartment of Psychiatry, University of Pittsburgh, Pittsburgh, PA 15213, USA.ORCID 0000-0002-8444-1385
Maïté CourelCNRS-UMR7622, Institut de Biologie Paris-Seine, Université Pierre & Marie Curie, 75252 Paris, France.ORCID 0000-0002-3215-4325
Robin J FreybergDepartment of Psychiatry, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Jonathan A JavitchDepartment of Psychiatry, Columbia University, New York, NY 10032, USA.ORCID 0000-0001-7395-2967
Zachary P WillsDepartment of Neurobiology, University of Pittsburgh, Pittsburgh, PA 15213, USA.ORCID 0000-0001-9822-6742
Estela Area-GomezDepartment of Neurology, Columbia University, New York, NY 10032, USA.
Sruti ShivaDepartment of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA 15261, USA.
Francesca BartoliniDepartment of Pathology and Cell Biology, Columbia University, New York, NY 10032, USA.
Allen VolchukProgram in Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada.
Sandra A MurrayDepartment of Cell Biology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Meir AridorDepartment of Cell Biology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Kenneth N FishDepartment of Psychiatry, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Peter WalterHHMI, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA.ORCID 0000-0002-6849-708X
Tamas BallaSection on Molecular Signal Transduction, Program for Developmental Neuroscience, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Deborah FassDepartment of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.ORCID 0000-0001-9418-6069
Sharon G WolfDepartment of Chemical Research Support, Weizmann Institute of Science, Rehovot, Israel.ORCID 0000-0002-5337-5063
Simon C WatkinsDepartment of Cell Biology, University of Pittsburgh, Pittsburgh, PA 15213, USA.ORCID 0000-0003-4092-1552
José María CarazoBiocomputing Unit, Centro Nacional de Biotecnología-CSIC, Darwin 3, Campus Universidad Autónoma, 28049 Madrid, Spain.ORCID 0000-0003-0788-8447
Grant J JensenHHMI, Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.ORCID 0000-0003-1556-4864
Joachim FrankDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Zachary FreybergDepartment of Psychiatry, University of Pittsburgh, Pittsburgh, PA 15213, USA.ORCID 0000-0001-6460-0118
University of Pittsburgh · USColumbia University · USNew York Psychoanalytic Society and Institute · USCalifornia Institute of Technology · USCentro Nacional de Biotecnología · ESNational Institute of Child Health · HUNew York Structural Biology Center · USUniversity of California, San Francisco · USWeizmann Institute of Science · ILCentre National de la Recherche Scientifique · FRColumbia University Irving Medical Center · USHospital for Sick Children · CA
Funding
Tumor Biology and Microenvironment ProgramP30CA013696 · NCI · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI Anil K Rustgi · 1985 to 2026
$115.3MX-ray Cryst. CoreP50GM082545 · NIGMS · UNIVERSITY OF UTAH · PI SUNDQUIST, WESLEY I. · 2007 to 2018
$51.1MZWITTERION SURFACTANT DDMAB FOR IMPROV STABIL OF PROTEINP41RR001209 · NCRR · STANFORD UNIVERSITY · PI SCOTT, EMILY E · 1985 to 2011
$38.9MPhosphoinositide-calcium Signaling In Cell RegulationZIAHD000196 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI BALLA, TAMAS · 2009 to 2025
$26.3MVirus TransmissionP50AI150464 · NIAID · UNIVERSITY OF UTAH · PI SUNDQUIST, WESLEY I. · 2019 to 2021
$14.9MTRD #3: An automated and streamlined pipeline for in-situ molecular microscopyP41GM103310 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2012 to 2021
$14.7MSTRUCTURE ANALYSIS OF MACROMOLECULAR ASSEMBLIESR01GM029169 · NIGMS · WADSWORTH CENTER · PI FRANK, JOACHIM · 1985 to 2019
$6.1MSTRUCTURAL ANALYSIS OF MACROMOLECULAR ASSEMBLYR37GM029169 · NIGMS · WADSWORTH CENTER · PI FRANK, JOACHIM · 1997 to 2006
$2.8MRole of C99 in the regulation of lipid metabolism- Relevance to Alzheimer's diseaseR01AG056387 · NIA · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI AREA GOMEZ, ESTELA · 2017 to 2021
$2.1MDual-Beam Scanning Electron Microscope for New York Structural Biology CenterS10RR029300 · NCRR · NEW YORK STRUCTURAL BIOLOGY CENTER · PI STOKES, DAVID L. · 2010 to 2010
$2.0MImaging large macromolecular complexes inside cells with electron cryotomographyR35GM122588 · NIGMS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI JENSEN, GRANT J · 2017 to 2020
$1.9MRevealing Novel Mechanisms of Amphetamine Action in a Drosophila ModelK08DA031241 · NIDA · NEW YORK STATE PSYCHIATRIC INSTITUTE DBA RESEARCH FOUNDATION FOR MENTAL HYGIENE, INC · PI FREYBERG, ZACHARY · 2011 to 2015
$931kHoward Hughes Medical InstituteNCI NIH HHS P30 CA013696NCRR NIH HHS P41 RR001209NCRR NIH HHS S10 RR019003NCRR NIH HHS S10 RR025488NCRR NIH HHS S10 RR029300NIAID NIH HHS P50 AI150464NIA NIH HHS K01 AG045335NIA NIH HHS R01 AG056387NIA NIH HHS R56 AG058593NIDA NIH HHS K08 DA031241NIGMS NIH HHS P41 GM103310NIGMS NIH HHS P50 GM082545NIGMS NIH HHS R01 GM029169NIGMS NIH HHS R35 GM122588NIGMS NIH HHS R37 GM029169NIH HHS S10 OD016236NINDS NIH HHS F30 NS093798
Abstract
The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo-electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic β-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We show that RAVs exist as distinct, highly dynamic structures separate from the intact ER reticular architecture that interact with mitochondria via direct intermembrane contacts. These findings describe a new ER subcompartment within cells.
Indexed as
AnimalsBiological TransportCryoelectron MicroscopyCytoplasmic VesiclesEndoplasmic ReticulumGolgi ApparatusMiceMitochondriaMolecular ImagingOrgan SpecificityRatsRibosomesStress, Physiological
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