ArticleMalaria journal2020
The response of Plasmodium falciparum to isoleucine withdrawal is dependent on the stage of progression through the intraerythrocytic cell cycle.
Article in Malaria journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Isoleucine absence from adult human hemoglobin is mirrored in mosquito proteomes and limits malaria parasite growth.bioRxiv : the preprint server for biology · 2026Article
- A guide to the apicomplexan cell cycle: endodyogeny and schizogony.Trends in parasitology · 2025Review
- Metabolomics Insights into Salivary Profile in Dogs withBiomolecules · 2025Article
- tRNA regulation and amino acid usage bias reflect a coordinated metabolic adaptation iniScience · 2024Article
- A novel type of serum‑free medium for culturing human airway epithelium cells.Experimental and therapeutic medicine · 2023Article
- Cripowellins PauseMolecules (Basel, Switzerland) · 2023Article
- Plasmodium schizogony, a chronology of the parasite's cell cycle in the blood stage.PLoS pathogens · 2023Review
- A non-canonical sensing pathway mediates Plasmodium adaptation to amino acid deficiency.Communications biology · 2023Article
- Characterization of Apicomplexan Amino Acid Transporters (ApiATs) in the Malaria Parasite Plasmodium falciparum.mSphere · 2021Article
- Amino Acid Metabolism in Apicomplexan Parasites.Metabolites · 2021Review
- How Many Is Enough? - Challenges of Multinucleated Cell Division in Malaria Parasites.Frontiers in cellular and infection microbiology · 2021Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundA previous study reported that the malaria parasite Plasmodium falciparum enters an altered growth state upon extracellular withdrawal of the essential amino acid isoleucine. Parasites slowed transit through the cell cycle when deprived of isoleucine prior to the onset of S-phase.
methodsThis project was undertaken to study at higher resolution, how isoleucine withdrawal affects parasite growth. Parasites were followed at regular intervals across an extended isoleucine deprivation time course across the cell cycle using flow cytometry.
resultsThese experiments revealed that isoleucine-deprived parasites never exit the cell cycle, but instead continuously grow at a markedly reduced pace. Moreover, slow growth occurs only if isoleucine is removed prior to the onset of schizogony. After S-phase commenced, the parasite is insensitive to isoleucine depletion and transits through the cell cycle at the normal pace.
conclusionsThe markedly different response of the parasite to isoleucine withdrawal before or after the onset of DNA replication is reminiscent of the nutrient-dependent G1 cell cycle checkpoints described in other organisms.
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