ArticleCell reports2020
Gene Regulatory and Expression Differences between Mouse and Pig Limb Buds Provide Insights into the Evolutionary Emergence of Artiodactyl Traits.
Article in Cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Functional architecture of cardiac TF regulatory landscapes in control of mammalian heart development.bioRxiv : the preprint server for biology · 2025Article
- Uncovering hidden enhancers through unbiased in vivo testing.Nature communications · 2025Article
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- Epigenomic signatures of cis-regulatory elements in the developing mouse and pig forelimb.Scientific data · 2025Article
- Single-cell profiling of penta- and tetradactyl mouse limb buds identifies mesenchymal progenitors controlling digit numbers and identities.Nature communications · 2025Article
- A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survival.Nature communications · 2024Article
- The developmental and evolutionary characteristics of transcription factor binding site clustered regions based on an explainable machine learning model.Nucleic acids research · 2024Article
- Insights into digit evolution from a fate map study of the forearm using Chameleon, a new transgenic chicken line.Development (Cambridge, England) · 2024Article
- Nile Tilapia (International journal of molecular sciences · 2024Article
- Article
- Topologically associating domain boundaries are required for normal genome function.Communications biology · 2023Article
- Investigating chromatin accessibility during development and differentiation by ATAC-sequencing to guide the identification of cis-regulatory elements.Biochemical Society transactions · 2022Article
- Enhancer-promoter interaction maps provide insights into skeletal muscle-related traits in pig genome.BMC biology · 2022Article
- Loxl2 and Loxl3 Paralogues Play Redundant Roles during Mouse Development.International journal of molecular sciences · 2022Article
- SMAD4 target genes are part of a transcriptional network that integrates the response to BMP and SHH signaling during early limb bud patterning.Development (Cambridge, England) · 2021Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2021Article
- (Non)Parallel developmental mechanisms in vertebrate appendage reduction and loss.Ecology and evolution · 2021Review
- Conserved and species-specific chromatin remodeling and regulatory dynamics during mouse and chicken limb bud development.Nature communications · 2021Article
- Spatial regulation by multiple Gremlin1 enhancers provides digit development with cis-regulatory robustness and evolutionary plasticity.Nature communications · 2021Article
- Non-model systems in mammalian forelimb evo-devo.Current opinion in genetics & development · 2021Review
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Digit loss/reductions are evolutionary adaptations in cursorial mammals such as pigs. To gain mechanistic insight into these processes, we performed a comparative molecular analysis of limb development in mouse and pig embryos, which revealed a loss of anterior-posterior polarity during distal progression of pig limb bud development. These alterations in pig limb buds are paralleled by changes in the mesenchymal response to Sonic hedgehog (SHH) signaling, which is altered upstream of the reduction and loss of Fgf8 expression in the ectoderm that overlaps the reduced and vestigial digit rudiments of the pig handplate, respectively. Furthermore, genome-wide open chromatin profiling using equivalent developmental stages of mouse and pig limb buds reveals the functional divergence of about one-third of the regulatory genome. This study uncovers widespread alterations in the regulatory landscapes of genes essential for limb development that likely contributed to the morphological diversion of artiodactyl limbs from the pentadactyl archetype of tetrapods.
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