ArticlePloS one2020
Comparison of sonication with chemical biofilm dislodgement methods using chelating and reducing agents: Implications for the microbiological diagnosis of implant associated infection.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Culture of periprosthetic tissue in blood culture bottles for diagnosing periprosthetic joint infection.BMC musculoskeletal disorders · 2019Pooled it
- Effect of sonication on staphylococcal and gram-negative biofilms relevant to prosthetic joint infections: an in vitro study.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- CApEsid biOfilm: a suggested pipeline for clinical phage microbiology for biofilm infections based on comparative method study.Scientific reports · 2026Article
- Differential antimicrobial responses in polymicrobial triple-species biofilms associated with persistent urinary tract infections.Scientific reports · 2026Article
- Novel lytic phages improve the antibiofilm activity of dalbavancin, daptomycin, and fosfomycin against vancomycin-resistant enterococci.Microbiology spectrum · 2026Article
- Hypertonic thermal solutions as a putative strategy for respiratory biofilm disruption.Open medicine (Warsaw, Poland) · 2026Article
- Unraveling novel insights into dual-species cariogenic biofilm formation on aged teeth: a comparative analysis on natural vs artificial bioengineered dentin models.Applied and environmental microbiology · 2025Article
- In vitro activity of cefiderocol against Gram-negative aerobic bacilli in planktonic and biofilm form-alone and in combination with bacteriophages.Scientific reports · 2025Article
- Advantages in orthopaedic implant infection diagnostics by additional analysis of explants.International orthopaedics · 2025Article
- Saponin Improves Recovery of Bacteria from Orthopaedic Implants for Enhanced Diagnosis Ex Vivo.Microorganisms · 2025Article
- In Vitro Bacterial Growth on Titanium Surfaces Treated with Nanosized Hydroxyapatite.Journal of functional biomaterials · 2025Article
- Antimicrobial Resistance Patterns and Biofilm Analysis via Sonication in Intensive Care Unit Patients at a County Emergency Hospital in Romania.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Phage Cocktail againstInternational journal of molecular and cellular medicine · 2025Article
- Advancing Diagnostic Techniques for Implant-Related Infections: the Synergistic Effect of Sonication and Dithiothreitol.Maedica · 2024Article
- Enhancing Pathogen Detection in Implant-Related Infections through Chemical Antibiofilm Strategies: A Comprehensive Review.Antibiotics (Basel, Switzerland) · 2024Review
- Role of Dithiothreitol in Detection of Orthopaedic Implant-Associated Infections.Journal of personalized medicine · 2024Article
- Effect of pH and hydroxyapatite-like layer formation on the antibacterial properties of borophosphate bioactive glass incorporated poly(methyl methacrylate) bone cement.Frontiers in bioengineering and biotechnology · 2024Article
- Article
- Sonication protocols and their contributions to the microbiological diagnosis of implant-associated infections: a review of the current scenario.Frontiers in cellular and infection microbiology · 2024Review
- Isothermal microcalorimetry improves accuracy and time to bacterial detection of periprosthetic joint infection after total joint arthroplasty.Journal of clinical microbiology · 2023Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diagnosis of implant-associated infections is hampered due to microbial adherence and biofilm formation on the implant surface. Sonication of explanted devices was shown to improve the microbiological diagnosis by physical removal of biofilms. Recently, chemical agents have been investigated for biofilm dislodgement such as the chelating agent ethylenediaminetetraacetic acid (EDTA) and the reducing agent dithiothreitol (DTT). We compared the activity of chemical methods for biofilm dislodgement to sonication in an established in vitro model of artificial biofilm. Biofilm-producing laboratory strains of Staphylococcus epidermidis (ATCC 35984), S. aureus (ATCC 43300), E. coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 53278) were used. After 3 days of biofilm formation, porous glass beads were exposed to control (0.9% NaCl), sonication or chemical agents. Quantitative and qualitative biofilm analyses were performed by colony counting, isothermal microcalorimetry and scanning electron microscopy. Recovered colony counts after treatment with EDTA and DTT were similar to those after exposure to 0.9% NaCl for biofilms of S. epidermidis (6.3 and 6.1 vs. 6.0 log10 CFU/mL, S. aureus (6.4 and 6.3 vs. 6.3 log10 CFU/mL), E. coli (5.2 and 5.1 vs. 5.1 log10 CFU/mL and P. aeruginosa (5.1 and 5.2 vs. 5.0 log10 CFU/mL, respectively). In contrast, with sonication higher CFU counts were detected with all tested microorganisms (7.5, 7.3, 6.2 and 6.5 log10 CFU/mL, respectively) (p <0.05). Concordant results were observed with isothermal microcalorimetry and scanning electron microscopy. In conclusion, sonication is superior to both tested chemical methods (EDTA and DTT) for dislodgement of S. epidermidis, S. aureus, E. coli and P. aeruginosa biofilms. Future studies may evaluate potential additive effect of chemical dislodgement to sonication.
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