Evidence map›Paper›PMID 32267667›Full record

ArticleACS nano2020

Big Is Beautiful: Enhanced saRNA Delivery and Immunogenicity by a Higher Molecular Weight, Bioreducible, Cationic Polymer.

Anna K Blakney, Yunqing Zhu, Paul F McKay, Clément R Bouton, Jonathan Yeow, Jiaqing Tang, Kai Hu, Karnyart Samnuan, Christopher L Grigsby, Robin J Shattock and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS nano, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.

0numbers the graph read from it
0cells of the map it votes in
69citing papers in PubMed
7.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

69 citing papers in PubMed, 150 citations in OpenAlex.

  1. Article
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  7. Immunology of RNA-based vaccines: The critical interplay between inflammation and expression.Molecular therapy : the journal of the American Society of Gene Therapy · 2025
    Review
  8. Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Alphaviral backbone of self-amplifying RNA enhances protein expression and immunogenicity against SARS-CoV-2 antigen.Molecular therapy : the journal of the American Society of Gene Therapy · 2025
    Article
  14. Review
  15. Review
  16. Review
  17. Review
  18. Review
  19. Article
  20. Review

9 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 3 countries.

Anna K BlakneyDepartment of Infectious Diseases, Imperial College London, Norfolk Place, London, W2 1PG, U.K.ORCID 0000-0002-5812-9689
Yunqing ZhuDepartment of Materials, Department of Bioengineering, Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, U.K.ORCID 0000-0002-2557-003X
Paul F McKayDepartment of Infectious Diseases, Imperial College London, Norfolk Place, London, W2 1PG, U.K.
Clément R BoutonDepartment of Infectious Diseases, Imperial College London, Norfolk Place, London, W2 1PG, U.K.
Jonathan YeowDepartment of Materials, Department of Bioengineering, Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, U.K.ORCID 0000-0003-3709-5149
Jiaqing TangDepartment of Materials, Department of Bioengineering, Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, U.K.
Kai HuDepartment of Infectious Diseases, Imperial College London, Norfolk Place, London, W2 1PG, U.K.
Karnyart SamnuanDepartment of Infectious Diseases, Imperial College London, Norfolk Place, London, W2 1PG, U.K.
Christopher L GrigsbyDepartment of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, 171 65, Sweden.
Robin J ShattockDepartment of Infectious Diseases, Imperial College London, Norfolk Place, London, W2 1PG, U.K.
Molly M StevensDepartment of Materials, Department of Bioengineering, Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, U.K.ORCID 0000-0002-7335-266X
Imperial College London · GBKarolinska Institutet · SETongji University · CN

Funding

Medical Research Council MC_PC_16046Medical Research Council MR/R015651/1Wellcome TrustWellcome Trust 204834/Z/16/Z
6 · The paper itself

Abstract

Self-amplifying RNA (saRNA) vaccines are highly advantageous, as they result in enhanced protein expression compared to mRNA (mRNA), thus minimizing the required dose. However, previous delivery strategies were optimized for siRNA or mRNA and do not necessarily deliver saRNA efficiently due to structural differences of these RNAs, thus motivating the development of saRNA delivery platforms. Here, we engineer a bioreducible, linear, cationic polymer called "pABOL" for saRNA delivery and show that increasing its molecular weight enhances delivery both

Indexed as

PolymersCationsMolecular WeightRNA, MessengerRNA, Small InterferingCationsPolymersRNA, MessengerRNA, Small Interferinginfluenzanucleic acidpolymerrepliconRNAvaccine

Identifiers

PMID32267667
PMCPMC7304921
OpenAlexW3014858505

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.