ArticleMicrobial biotechnology2020
Pseudomonas putida KT2440 is naturally endowed to withstand industrial-scale stress conditions.
Article in Microbial biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 76 citations in OpenAlex.
- Enhancing acetate-based biosurfactant production by Pseudomonas putida KT2440 through fermentation optimization.Microbial cell factories · 2026Article
- Characterisation of metabolic burden in Pseudomonas putida reveals precursor limitation in heterologous lycopene production.Microbial cell factories · 2026Article
- Cascading recombinase memory switch for programmable and stable gene expression in Pseudomonas putida.Nucleic acids research · 2026Article
- Pan-Genomic and Phenotypic Characterisation of Petroleum Hydrocarbon Degradation by Pseudomonas Species.Environmental microbiology reports · 2026Review
- Scaling-up the Bioconversion of Lignin to 2,4-Pyridinedicarboxylic Acid With Engineered Pseudomonas putida for Bio-Based Plastics Production.Biotechnology and bioengineering · 2025Article
- Protein-Protein Complex Stability Controls Substrate Scope in a β-Ketoacyl-ACP Reductase Specific for Medium Chains.Angewandte Chemie (International ed. in English) · 2025Article
- Chicken Manure as a Sustainable Bile Acid Source for Biotechnological Applications.Microbial biotechnology · 2025Article
- Lignin-Degrading Enzymes and the Potential ofMicroorganisms · 2025Review
- Bioprocess exploitation of microaerobic auto-induction using the example of rhamnolipid biosynthesis in Pseudomonas putida KT2440.Journal of biological engineering · 2025Article
- Exploring the Metabolic Response of Pseudomonas putida to L-arginine.Advances in experimental medicine and biology · 2025Article
- Growth Characteristics ofMycobiology · 2025Article
- Food for Thoughts for Prospective Biomanufacturing.Microbial biotechnology · 2025Article
- Enhanced biosynthesis of poly(3-hydroxybutyrate) in engineered strains of Pseudomonas putida via increased malonyl-CoA availability.Microbial biotechnology · 2024Article
- Systems biology of electrogenic Pseudomonas putida - multi-omics insights and metabolic engineering for enhanced 2-ketogluconate production.Microbial cell factories · 2024Article
- Physiology, fast and slow: bacterial response to variable resource stoichiometry and dilution rate.mSystems · 2024Article
- Review
- Reliable Genomic Integration Sites inACS synthetic biology · 2024Article
- Single-cell RNA sequencing reveals plasmid constrains bacterial population heterogeneity and identifies a non-conjugating subpopulation.Nature communications · 2024Article
- A Genetically Encoded Biosensor for the Detection of Levulinic Acid.Journal of microbiology and biotechnology · 2023Article
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas putida is recognized as a very promising strain for industrial application due to its high redox capacity and frequently observed tolerance towards organic solvents. In this research, we studied the metabolic and transcriptional response of P. putida KT2440 exposed to large-scale heterogeneous mixing conditions in the form of repeated glucose shortage. Cellular responses were mimicked in an experimental setup comprising a stirred tank reactor and a connected plug flow reactor. We deciphered that a stringent response-like transcriptional regulation programme is frequently induced, which seems to be linked to the intracellular pool of 3-hydroxyalkanoates (3-HA) that are known to serve as precursors for polyhydroxyalkanoates (PHA). To be precise, P. putida is endowed with a survival strategy likely to access cellular PHA, amino acids and glycogen in few seconds under glucose starvation to obtain ATP from respiration, thereby replenishing the reduced ATP levels and the adenylate energy charge. Notably, cells only need 0.4% of glucose uptake to build those 3-HA-based energy buffers. Concomitantly, genes that are related to amino acid catabolism and β-oxidation are upregulated during the transient absence of glucose. Furthermore, we provide a detailed list of transcriptional short- and long-term responses that increase the cellular maintenance by about 17% under the industrial-like conditions tested.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.