ArticleJournal of experimental botany2020
Biological nitrogen fixation in maize: optimizing nitrogenase expression in a root-associated diazotroph.
Article in Journal of experimental botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 87 citations in OpenAlex.
- Engineered diazotrophs with host-inducible nitrogen supply systems: Transforming rice farming through innovative nitrogen biofertilizers.Journal of integrative plant biology · 2026Review
- Isolation and Genetic Enhancement of Nitrogen-Fixing Rhizobacteria for Promoting Growth in Maize.Microorganisms · 2026Article
- The coupling effect ofSynthetic and systems biotechnology · 2026Article
- Hyperspectral reporters for long-distance and wide-area detection of gene expression in living bacteria.Nature biotechnology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Rhizobia mitigates autotoxicity inFrontiers in microbiology · 2026Article
- Unlocking associative nitrogen fixation in Poaceae: molecular mechanisms, regulatory constraints, and pathways to sustainable productivity.Frontiers in microbiology · 2026Review
- Soil Carbon and Organic Matter Fractions Under Nitrogen Management in a Maize-Soybean-Cover Crop System in the Cerrado.Plants (Basel, Switzerland) · 2025Article
- Second Genome: Rhizosphere Microbiome as a Key External Driver of Nitrogen Use Efficiency in Maize.Plants (Basel, Switzerland) · 2025Review
- Wild rice-associatedmSystems · 2025Article
- Nitrogen Use Efficiency in Agriculture: Integrating Biotechnology, Microbiology, and Novel Delivery Systems for Sustainable Agriculture.Plants (Basel, Switzerland) · 2025Review
- Transgene expression and root hair deformation of transgenic rice plants harbouring legume-specific Nod factor receptor genes in the presence and absence of nitrogen when inoculated with Sinorhizobium meliloti.Molecular biology reports · 2025Article
- A roadmap to understanding and anticipating microbial gene transfer in soil communities.Microbiology and molecular biology reviews : MMBR · 2025Review
- Microbiome Migration from Soil to Leaves in Maize and Rice.Microorganisms · 2025Article
- Environmental Applications of GM Microorganisms: Tiny Critters Posing Huge Challenges for Risk Assessment and Governance.International journal of molecular sciences · 2025Review
- Design and regulation of engineered bacteria for environmental release.Nature microbiology · 2025Review
- Synthetic communities as a model for determining interactions between a biofertilizer chassis organism and native microbial consortia.The ISME journal · 2025Article
- Biotechnology executive order opens door for regulatory reform and social acceptance of genetically engineered microbes in agriculture.GM crops & food · 2024Article
- Bacterial and fungal root endophytes alter survival, growth, and resistance to grazing in a foundation plant species.Oecologia · 2024Article
- Exploring and exploiting the rice phytobiome to tackle climate change challenges.Plant communications · 2024Review
Corrections and comments
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Authors and funding
20 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants depend upon beneficial interactions between roots and root-associated microorganisms for growth promotion, disease suppression, and nutrient availability. This includes the ability of free-living diazotrophic bacteria to supply nitrogen, an ecological role that has been long underappreciated in modern agriculture for efficient crop production systems. Long-term ecological studies in legume-rhizobia interactions have shown that elevated nitrogen inputs can lead to the evolution of less cooperative nitrogen-fixing mutualists. Here we describe how reprogramming the genetic regulation of nitrogen fixation and assimilation in a novel root-associated diazotroph can restore ammonium production in the presence of exogenous nitrogen inputs. We isolated a strain of the plant-associated proteobacterium Kosakonia sacchari from corn roots, characterized its nitrogen regulatory network, and targeted key nodes for gene editing to optimize nitrogen fixation in corn. While the wild-type strain exhibits repression of nitrogen fixation in conditions replete with bioavailable nitrogen, such as fertilized greenhouse and field experiments, remodeled strains show elevated levels in the rhizosphere of corn in the greenhouse and field even in the presence of exogenous nitrogen. Such strains could be used in commercial applications to supply fixed nitrogen to cereal crops.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.