Using Environmental Sampling Techniques to Conduct Influenza A Virus Surveillance in Poultry and Waterfowl at Ohio Agricultural Exhibitions.

The outbreak of highly pathogenic H5Nx influenza A viruses (IAVs) in the United States during 2014-2015 caused devastating economic losses; therefore, several measures were established to control and eliminate highly pathogenic H5Nx from U.S. poultry flocks. One such measure was a temporary ban on poultry exhibitions during 2015, and this decision dramatically affected youth raising poultry as part of agricultural education programs. During the summer of 2016, surveillance of the environment was conducted at 20 Ohio agricultural fairs to estimate the prevalence of IAV in exhibition poultry to determine the baseline during nonoutbreak exhibition seasons. Of the 400 total samples collected, two were positive by real-time reverse transcription-PCR; however, virus isolation attempts with both embryonating chicken eggs and cell culture were unsuccessful. The detection of nucleic acid highlights the risk exhibition poultry could play in the transmission and spread of IAVs between humans, swine, wild birds, and domestic poultry during low or highly pathogenic IAV outbreaks. Additional surveillance at agricultural fairs and biosecurity education for youth exhibitors in this setting are warranted to reduce risk.