ArticleBioprocess and biosystems engineering2020
Pre-stage perfusion and ultra-high seeding cell density in CHO fed-batch culture: a case study for process intensification guided by systems biotechnology.
Article in Bioprocess and biosystems engineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 55 citations in OpenAlex.
- Impact of N-1 phase media optimization as a bioprocess intensification strategy on fed-batch production.Bioprocess and biosystems engineering · 2026Article
- Multivariate analysis of the effects of high seeding density on therapeutic protein production and sialylation in an intensified CHO cell culture process.Bioprocess and biosystems engineering · 2026Article
- Accelerated dynamic characterization of CHO semi-perfusion processes using intensified design of experiments.Bioprocess and biosystems engineering · 2026Article
- Developing affordable monoclonal antibodies for LMICs through high performance manufacturing processes and LMIC based commercial manufacturing.Frontiers in pediatrics · 2026Article
- Enhancing monoclonal antibody production efficiency using CHO-MK cells and specific media in a conventional fed-batch culture.Cytotechnology · 2025Article
- Cell specific perfusion rates drive growth dynamics and metabolism in CHO N-1 perfusion processes independent of perfusion rate control method.Frontiers in bioengineering and biotechnology · 2025Article
- Perfusion process with tangential flow filtration for oncolytic VSV-GP production.Frontiers in bioengineering and biotechnology · 2025Article
- A high-density microfluidic bioreactor for the automated manufacturing of CAR T cells.Nature biomedical engineering · 2024Article
- Impact of fed-batch process intensification on the productivity and product quality of two CHO cell lines expressing unique novel molecular format proteins.Bioprocess and biosystems engineering · 2024Article
- Deciphering Metabolic Pathways in High-Seeding-Density Fed-Batch Processes for Monoclonal Antibody Production: A Computational Modeling Perspective.Bioengineering (Basel, Switzerland) · 2024Article
- Effects of process intensification on homogeneity of an IgG1:κ monoclonal antibody during perfusion culture.Applied microbiology and biotechnology · 2024Article
- Triple Space-Time Yield in Discontinuous Antibody Biomanufacturing by Combination of Synergetic Process Intensification Strategies.Bioengineering (Basel, Switzerland) · 2023Article
- Automated Raman feed-back control of multiple supplemental feeds to enable an intensified high inoculation density fed-batch platform process.Bioprocess and biosystems engineering · 2023Article
- Transcriptomic analysis reveals mode of action of butyric acid supplementation in an intensified CHO cell fed-batch process.Biotechnology and bioengineering · 2022Article
- N-1 Perfusion Platform Development Using a Capacitance Probe for Biomanufacturing.Bioengineering (Basel, Switzerland) · 2022Article
- Exploring the limits of conventional small-scale CHO fed-batch for accelerated on demand monoclonal antibody production.Bioprocess and biosystems engineering · 2022Article
- Rapid intensification of an established CHO cell fed-batch process.Biotechnology progress · 2022Article
- Generic and specific recurrent neural network models: Applications for large and small scale biopharmaceutical upstream processes.Biotechnology reports (Amsterdam, Netherlands) · 2021Article
- Application of metabolic modeling for targeted optimization of high seeding density processes.Biotechnology and bioengineering · 2021Article
- Digital Seed Train Twins and Statistical Methods.Advances in biochemical engineering/biotechnology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Process intensification strategies are needed in the field of therapeutic protein production for higher productivities, lower cost of goods and improved facility utilization. This work describes an intensification approach, which connects a tangential-flow-filtration (TFF) based pre-stage perfusion process with a concentrated fed-batch production culture inoculated with an ultra-high seeding density (uHSD). This strategy shifted biomass production towards the pre-stage, reaching up to 45 × 10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.