ArticleFrontiers in plant science2020
Efficient Generation of CRISPR/Cas9-Mediated Homozygous/Biallelic
Article in Frontiers in plant science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- Optimized protocol for efficient generation, confirmation, transformation, and CRISPR editing of grapevine hairy roots.Journal of experimental botany · 2026Article
- Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Advances in gene editing for legume improvement: technologies, progress, and prospects.Frontiers in genome editing · 2026Review
- High-Frequency Generation of Homozygous/Biallelic Mutants via CRISPR/Cas9 Driven byInternational journal of molecular sciences · 2025Article
- Applying conventional and cell-type-specific CRISPR/Cas9 genome editing in legume plants.aBIOTECH · 2025Review
- Genome editing for grass improvement and future agriculture.Horticulture research · 2025Article
- CRISPR/Cas9-Mediated Generation of Mutant Lines inBiology · 2024Article
- Callus Induction Followed by Regeneration and Hairy Root Induction in Common Buckwheat.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Biochemical analysis of the TPS-a subfamily inFrontiers in plant science · 2024Article
- Targeted mutagenesis of Medicago truncatula Nodule-specific Cysteine-Rich (NCR) genes using the Agrobacterium rhizogenes-mediated CRISPR/Cas9 system.Scientific reports · 2023Article
- Induction and metabolomic analysis of hairy roots of Atractylodes lancea.Applied microbiology and biotechnology · 2023Article
- Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein and hairy roots: a perfect match for gene functional analysis and crop improvement.Current opinion in biotechnology · 2023Review
- Progress and Challenges in Elucidating the Functional Role of Effectors in the Soybean-Journal of fungi (Basel, Switzerland) · 2022Review
- Application of CRISPR/Cas9 System for Efficient Gene Editing in Peanut.Plants (Basel, Switzerland) · 2022Article
- CRISPR/Cas9-Mediated Mutagenesis of the Granule-Bound Starch Synthase Gene in the Potato Variety Yukon Gold to Obtain Amylose-Free Starch in Tubers.International journal of molecular sciences · 2022Article
- Highly efficientFrontiers in plant science · 2022Article
- Gene-Editing Technologies and Applications in Legumes: Progress, Evolution, and Future Prospects.Frontiers in genetics · 2022Review
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- Approach for in vivo delivery of CRISPR/Cas system: a recent update and future prospect.Cellular and molecular life sciences : CMLS · 2021Review
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the process of acquiring mutants mediated by CRISPR/Cas9, plantlets are often regenerated from both mutated and non-mutated cells in a random manner, which increase the odds of chimeric mutated plant. In general, it's necessary to infect more explants or grow to next generation for the need of generating more biallelic or homozygous mutants. In present study, an efficient way of obtaining biallelic or homozygous mutated lines via fast-growing hairy root system without increasing numbers of infected explants or prolonging sexual propagation generation is reported. The fast growing lateral branches of hair roots are originated deep within the parental root from a small number of founder cells at the periphery, and therefore were employed as a library that classify different editing types in different lateral branches in which the homozygous or biallelic lines were screened. Here,
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.