ArticleParasites & vectors2020
Transovarial transmission of Borrelia spp., Rickettsia spp. and Anaplasma phagocytophilum in Ixodes ricinus under field conditions extrapolated from DNA detection in questing larvae.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
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Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it, 80 citations in OpenAlex.
- Prevalence and associated factors of Anaplasma, Rickettsia, and Coxiella-related bacteria in ticks in China: A systematic review and meta-analysis.Experimental & applied acarology · 2026Pooled it
- Immuno-Informatics Design of Multi-Epitope Vaccine Candidate AgainstHealth science reports · 2026Article
- Prevalence and Species Diversity of Spotted Fever Group Rickettsiae in Ixodid Ticks Collected in Northwest Russia.Tropical medicine and infectious disease · 2026Article
- Identification and mapping of potential and highly likely vectors for selected vector-borne diseases in the EU and neighbouring countries.EFSA journal. European Food Safety Authority · 2026Article
- A Bayesian modelling framework for estimating tick-borne pathogen transmission dynamics at the host-tick interface.PLoS computational biology · 2026Article
- The Lyme Disease Vaccine Paradox.Journal of clinical medicine · 2026Article
- From reservoirs to ecological integrators: the role of EuropeanFrontiers in veterinary science · 2026Review
- Molecular and serological evidence of spotted fever group rickettsiae and Borrelia co-infection in Ixodes ricinus and humans in north-eastern Poland.Scientific reports · 2025Article
- Identification and pathogen screening of ectoparasites from companion animals in urban Vientiane, Lao PDR.PLoS neglected tropical diseases · 2025Article
- First Detection ofMicroorganisms · 2025Article
- Article
- There Goes the Neighbourhood-A Multi-City Study Reveals Ticks and Tick-Borne Pathogens Commonly Occupy Urban Green Spaces.Zoonoses and public health · 2025Article
- Molecular detection of Anaplasma phagocytophilum in field-collected Haemaphysalis larvae in the Republic of Korea.Parasites & vectors · 2025Article
- First evidence of transovarial transmission of Kyasanur Forest disease virus in Haemaphysalis and Rhipicephalus ticks in the wild.Parasites & vectors · 2025Article
- Molecular identification, genotyping and phylogenetic analysis ofFrontiers in microbiology · 2025Article
- Assessing zoonotic risk in a fenced natural park in northwestern Italy: integrating camera traps for a vector-host approach to investigate tick-borne pathogens.Frontiers in veterinary science · 2025Article
- Wide spectrum of tick-borne pathogens in juvenile Ixodes ricinus collected from autumn-migrating birds in the Vistula River Valley, Poland.BMC veterinary research · 2024Article
- Molecular epidemiological study on tick-borne pathogens in Qinghai Province, Northwestern China.Biosafety and health · 2024Article
- Pathogens detected in ticks (Ixodes ricinus) feeding on red squirrels (Sciurus vulgaris) from city parks in Warsaw.Experimental & applied acarology · 2024Article
- Review
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIxodes ricinus constitutes the main European vector tick for the Lyme borreliosis pathogen Borrelia burgdorferi (sensu lato), the relapsing fever borrelia Borrelia miyamotoi, as well as Anaplasma phagocytophilum and several Rickettsia species. Under laboratory conditions, a transovarial transmission to the next tick generation is described for Rickettsia spp. and Borrelia spp., especially regarding B. miyamotoi, whereas the efficiency of transovarial transfer under field conditions is largely unstudied.
methodsIn order to better estimate the potential infection risk by tick larvae for humans and animals, 1500 I. ricinus larvae from 50 collected "nests" (larvae adhering to the flag in a clumped manner) were individually examined for Borrelia, Rickettsia and A. phagocytophilum DNA using quantitative real-time PCR (qPCR).
resultsThirty-nine of 50 nests each (78.0%, 95% CI: 64.0-88.5%) were positive for Borrelia spp. and Rickettsia spp. DNA, and in three nests (6.0%, 95% CI: 1.3-16.5%) A. phagocytophilum DNA was detected. Overall, DNA from at least one pathogen could be detected in 90.0% (45/50, 95% CI: 78.2-96.7%) of the nests. Of the 1500 larvae, 137 were positive for Borrelia spp. DNA (9.1%, 95% CI: 7.7-10.7%), 341 for Rickettsia spp. DNA (22.7%, 95% CI: 20.6-24.9%) and three for A. phagocytophilum DNA (0.2%, 95% CI: 0-0.6%). Quantity of Borrelia spp. and Anaplasma spp. DNA in positive larvae was low, with 2.7 × 10
conclusionsThis study provides evidence for transovarial transmission of Rickettsia spp. and Borrelia spp. in I. ricinus under field conditions, possibly facilitating pathogen persistence in the ecosystem and reducing the dependence on the presence of suitable reservoir hosts. Further studies are needed to prove transovarial transmission and to explain the surprisingly high proportion of nests containing Rickettsia and/or Borrelia DNA-positive larvae compared to infection rates in adult ticks commonly reported in other studies.
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