ArticleJournal of materials chemistry. B2016
Dual-crosslinked hydrogel microwell system for formation and culture of multicellular human adipose tissue-derived stem cell spheroids.
Article in Journal of materials chemistry. B, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Micro Pattern-Based 3D Cell Culture Platform: An Overview of Technologies and Applications.Exploration (Beijing, China) · 2026Review
- Polymer design of microwell hydrogels influences epithelial-mesenchymal interactions during human bronchosphere formation.Advanced nanobiomed research · 2025Article
- Smart alginate inks for tissue engineering applications.Materials today. Bio · 2023Review
- Applications and Advances of Multicellular Tumor Spheroids: Challenges in Their Development and Analysis.International journal of molecular sciences · 2023Review
- Jammed Micro-Flake Hydrogel for Four-Dimensional Living Cell Bioprinting.Advanced materials (Deerfield Beach, Fla.) · 2022Article
- Automated Assessment of Cancer Drug Efficacy On Breast Tumor Spheroids in Aggrewell™400 Plates Using Image Cytometry.Journal of fluorescence · 2022Article
- 3D Cell Culture Systems: Tumor Application, Advantages, and Disadvantages.International journal of molecular sciences · 2021Review
- Fabrication approaches for high-throughput and biomimetic disease modeling.Acta biomaterialia · 2021Review
- Mesenchymal stem cells loaded on 3D-printed gradient poly(ε-caprolactone)/methacrylated alginate composite scaffolds for cartilage tissue engineering.Regenerative biomaterials · 2021Article
- Recent Advances in Three-Dimensional Multicellular Spheroid Culture and Future Development.Micromachines · 2021Review
- Comparison of the formation, adipogenic maturation, and retention of human adipose-derived stem cell spheroids in scaffold-free culture techniques.Journal of biomedical materials research. Part B, Applied biomaterials · 2020Article
- Rapid fabrication of sieved microwells and cross-flow microparticle trapping.Scientific reports · 2020Article
- Self-Assemblable Polymer Smart-Blocks for Temperature-Induced Injectable Hydrogel in Biomedical Applications.Frontiers in chemistry · 2020Review
- High-throughput approaches for screening and analysis of cell behaviors.Biomaterials · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Three-dimensional (3D) multicellular spheroids of human adipose tissue-derived stem cells (hASCs) are an attractive system for basic science studies and tissue engineering applications, as they can resemble cellular condensations present in developmental and healing processes. The purpose of this study was to engineer a hydrogel-based microwell platform by capitalizing on the differential swelling behavior of micropatterned dual-crosslinked oxidized, methacrylated alginate (OMA)/multi-arm polyethylene glycol (PEG) hydrogels for rapid formation of uniform multicellular hASC spheroids with controllable size and evaluation of the capacity of the system to be used to drive osteogenic differentiation of the spheroids. By changing the micropattern size, the size of the hydrogel microwells was easily controllable. Microwell-seeded hASCs generated spheroids of relatively uniform size and high cell viability. hASC spheroids exhibited rapid mineralization in osteogenic media, which was faster than that of conventional two-dimensionally cultured hASCs. This new hydrogel microwell system has great potential for controlled multicellular spheroid formation and defined signal presentation from the hydrogel material to the cell aggregates to regulate tissue formation.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.