ArticleMethods and protocols2020
An Optimised Step-by-Step Protocol for Measuring Relative Telomere Length.
Article in Methods and protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 82 citations in OpenAlex.
- IMSI does not improve pregnancy outcomes and live birth rate in severe teratozoospermia patients: a double-blind randomized controlled trial.Scientific reports · 2026Trial
- Shorter Leukocyte telomere length as a marker of biological aging in relapsing-remitting multiple sclerosis.Acta neurologica Belgica · 2026Article
- Human Islet miR-199a-5p and miR-214-3p Associate With Donor Age, BMI, and Sex and Mediate Cellular Aging in Islet-Derived Cells.Acta physiologica (Oxford, England) · 2026Article
- Characterization and Validation of Long-Term Cultured TERT-Immortalized Human Wharton's Jelly-Derived Mesenchymal Stromal Cells.Stem cell reviews and reports · 2026Article
- Senescence modulated cardiac repair with pluripotent stem cell-derived cardiomyocytes.Stem cell research & therapy · 2026Article
- Detection of Longer Leukocyte Telomere Length in Patients With Bone Sarcomas.World journal of oncology · 2026Article
- Relative leukocyte telomere length and the risk of lung cancer progression in individuals with pulmonary nodules.BMC medicine · 2026Article
- Improved Step-by-Step qPCR Method for Absolute Telomere Length Measurement.Methods and protocols · 2026Article
- Clinical outcome of an SCNT-derivedFrontiers in genome editing · 2026Article
- Significant shortening of leukocyte telomeres one year after childbirth in women with type 1 diabetes mellitus: a prospective cohort study.Frontiers in clinical diabetes and healthcare · 2026Article
- Repurposing MK0873 as a non-covalent hTERT-Targeting compound: a proof of concept study.Frontiers in aging · 2026Article
- A comparison of sperm parameters DNA fragmentation and telomere length in testicular versus ejaculated spermatozoa.Scientific reports · 2025Article
- Article
- A multi-omics investigation of sarcopenia and frailty: Integrating genomic, epigenomic and telomere length data.Experimental physiology · 2025Article
- POT1 genetic testing in melanoma-prone families in Sweden: germline variant prevalence and tumor spectrum in identified carriers.Acta oncologica (Stockholm, Sweden) · 2025Article
- Genetics Influences Telomere Length in Parkinson's Disease: A Study in Monozygotic Discordant Twins.Movement disorders : official journal of the Movement Disorder Society · 2025Article
- Birth of India's first cloned cattle and analysis of its health and reproduction status: A case study.Scientific reports · 2025Article
- Determinants of temporal change in telomere length and its associations with chronic complications and mortality in type 2 diabetes: the Fremantle diabetes study phase II.Cardiovascular diabetology · 2025Observational
- Cardiac remodelling, recognition memory deficits and accelerated ageing in a rat model of gestational diabetes.Diabetologia · 2025Article
- Shortened Relative Leukocyte Telomere Length Is Associated With Polycystic Ovary Syndrome and Metabolic Traits.Endocrinology, diabetes & metabolism · 2025Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Telomeres represent the nucleotide repeat sequences at the ends of chromosomes and are essential for chromosome stability. They can shorten at each round of DNA replication mainly because of incomplete DNA synthesis of the lagging strand. Reduced relative telomere length is associated with aging and a range of disease states. Different methods such as terminal restriction fragment analysis, real-time quantitative PCR (qPCR) and fluorescence in situ hybridization are available to measure telomere length; however, the qPCR-based method is commonly used for large population-based studies. There are multiple variations across qPCR-based methods, including the choice of the single-copy gene, primer sequences, reagents, and data analysis methods in the different reported studies so far. Here, we provide a detailed step-by-step protocol that we have optimized and successfully tested in the hands of other users. This protocol will help researchers interested in measuring relative telomere lengths in cells or across larger clinical cohort/study samples to determine associations of telomere length with health and disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.