Evidence map›Paper›PMID 32258125›Full record

Trial reportBioMed research international2020

Gene Expression Analysis of Human Papillomavirus-Associated Colorectal Carcinoma.

Qiancheng Qiu, Yazhen Li, Zhiqiang Fan, Fen Yao, Wenjun Shen, Jiayu Sun, Yumeng Yuan, Jinghong Chen, Leshan Cai, Yanxuan Xie and 3 more

Open access · hybridAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in BioMed research international, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 2 countries.

Qiancheng QiuThe First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China.
Yazhen LiDepartment of Cell Biology and Genetics, Shantou University Medical College, Shantou, Guangdong 515041, China.
Zhiqiang FanDepartment of Cell Biology and Genetics, Shantou University Medical College, Shantou, Guangdong 515041, China.
Fen YaoDepartment of Pharmacology, Shantou University Medical College, Shantou, Guangdong 515041, China.
Wenjun ShenDepartment of Cell Biology and Genetics, Shantou University Medical College, Shantou, Guangdong 515041, China.
Jiayu SunDepartment of Cell Biology and Genetics, Shantou University Medical College, Shantou, Guangdong 515041, China.
Yumeng YuanDepartment of Cell Biology and Genetics, Shantou University Medical College, Shantou, Guangdong 515041, China.
Jinghong ChenCenter for Disease Control and Prevention of Shantou, Guangdong 515041, China.ORCID https://orcid.org/0000-0002-1764-4748
Leshan CaiThe First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China.
Yanxuan XieThe First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China.
Kaixi LiuShantou Central Hospital, Shantou, Guangdong 515041, China.
Xiang ChenThe First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China.
Xiaoyang JiaoDepartment of Cell Biology and Genetics, Shantou University Medical College, Shantou, Guangdong 515041, China.ORCID https://orcid.org/0000-0002-7568-0595
Shantou University · CNShantou Central Hospital · CNTaiwan Centers for Disease Control · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeHuman papillomavirus (HPV) antigens had been found in colorectal cancer (CRC) tissue, but little evidence demonstrates the association of HPV with oncogene mutations in CRC. We aim to elucidate the mutated genes that link HPV infection and CRC carcinogenesis.

methodsCancerous and adjacent noncancerous tissues were obtained from CRC patients. HPV antigen was measured by using the immunohistochemical (IHC) technique. The differentially expressed genes (DEGs) in HPV-positive and HPV-negative tumor tissues were measured by using TaqMan Array Plates. The target genes were validated with the qPCR method.

results15 (31.9%) cases of CRC patients were observed to be HPV positive, in which HPV antigen was expressed in most tumor tissues rather than in adjacent noncancerous tissues. With TaqMan Array Plates analyses, we found that 39 differentially expressed genes (DEGs) were upregulated, while 17 DEGs were downregulated in HPV-positive CRC tissues compared with HPV-negative tissues. Four DEGs (MMP-7, MYC, WNT-5A, and AXIN2) were upregulated in tumor vs. normal tissues, or adenoma vs. normal tissue in TCGA, which was overlapped with our data. In the confirmation test, MMP-7, MYC, WNT-5A, and AXIN2 were upregulated in cancerous tissue compared with adjacent noncancerous tissue. MYC, WNT-5A, and AXIN2 were shown to be upregulated in HPV-positive CRC tissues when compared to HPV-negative tissues.

conclusionHPV-encoding genome may integrate into the tumor genomes that involved in multiple signaling pathways. Further genomic and proteomic investigation is necessary for obtaining a more comprehensive knowledge of signaling pathways associated with the CRC carcinogenesis.

Indexed as

Colorectal NeoplasmsGene Expression Regulation, NeoplasticPapillomavirus InfectionsAdultAgedAged, 80 and overFemaleHumansMaleMiddle AgedNeoplasm ProteinsPapillomaviridaeNeoplasm Proteins

Identifiers

PMID32258125
PMCPMC7103040
OpenAlexW3010855079

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.