ArticleGrana2020
The single-grain method: adding TEM to the equation.
Article in Grana, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed.
- Trimerous magnoliid flowers with a unique set of floral and pollen traits from the Late Cretaceous of Southern Bohemia (Czech Republic).The New phytologist · 2026Article
- Ultrastructural Analysis ofPlants (Basel, Switzerland) · 2025Article
- 24 million years of pollination interaction between European linden flowers and bumble bees.The New phytologist · 2025Article
- Morphology and ultrastructure of Tilioideae pollen: how to differentiate Craigia, Mortoniodendron, and Tilia.Botanical studies · 2025Article
- Barremian tricolpate pollen from Portugal-New evidence for the age of eudicot-related angiosperms.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Origin and Early Evolution of Hydrocharitaceae and the Ancestral Role ofPlants (Basel, Switzerland) · 2024Article
- Article
- How to extract and analyze pollen from internal organs and exoskeletons of fossil insects?STAR protocols · 2021Article
- Article
- Methodologies and Considerations in Evaluating Heat Stress Response and Thermotolerance of Pollen Grains.Physiologia plantarumReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
An advanced protocol to prepare single extant and fossil pollen grains for transmission electron microscopy (TEM) analysis allows for the fast recovery of data on the ultrastructure of pollen/spores. The protocol is easy to apply and less time consuming than previous methods. The 'loss' of pollen grains and pollen that is 'difficult to locate' within the embedding material is avoided, and each single pollen grain can be prepared successfully for TEM analysis. This preparation method is meant as an addition to the single-grain method using combined light and scanning electron microscopy to investigate dispersed fossil pollen grains developed by Dr Reinhard Zetter in the late 1980s.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.