Polarization-Based Sensing with a Self-Referenced Sample.

Joseph R Lakowicz, Ignacy Gryczynski, Zygmunt Gryczynski, Leah Tolosa, Jonathan D Dattelbaum, Govind Rao

Abstract read

In one paragraph

Article in Applied spectroscopy, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it

0cells of the map it votes in

7citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

Nanostructure-Engineered Optical and Electrochemical Biosensing Toward Food Safety Assurance.Foods (Basel, Switzerland) · 2025
Review
Detection of hyaluronidase activity using fluorescein labeled hyaluronic acid and Fluorescence Correlation Spectroscopy.Journal of photochemistry and photobiology. B, Biology · 2012
Article
Proteins from extremophiles as stable tools for advanced biotechnological applications of high social interest.Journal of the Royal Society, Interface · 2007
Review
On the Possibility of Glucose Sensing Using Boronic Acid and a Luminescent Ruthenium Metal-Ligand Complex.Journal of fluorescence · 2002
Article
A novel fluorescence competitive assay for glucose determinations by using a thermostable glucokinase from the thermophilic microorganism Bacillus stearothermophilus.Analytical biochemistry · 2002
Article
Optical determination of glutamine using a genetically engineered protein.Analytical biochemistry · 2001
Article
Novel fluorescence sensing methods for high throughput screening.Journal of biomolecular screening · 2000
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Joseph R LakowiczDepartment of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201 (J.R.L., I.G., Z.G., L.T., J.D.D.); and Medical Biotechnology Center and Department of Chemical and Biochemical Engineering, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201 (G.R.).

Ignacy GryczynskiDepartment of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201 (J.R.L., I.G., Z.G., L.T., J.D.D.); and Medical Biotechnology Center and Department of Chemical and Biochemical Engineering, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201 (G.R.).

Zygmunt GryczynskiDepartment of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201 (J.R.L., I.G., Z.G., L.T., J.D.D.); and Medical Biotechnology Center and Department of Chemical and Biochemical Engineering, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201 (G.R.).

Leah TolosaDepartment of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201 (J.R.L., I.G., Z.G., L.T., J.D.D.); and Medical Biotechnology Center and Department of Chemical and Biochemical Engineering, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201 (G.R.).

Jonathan D DattelbaumDepartment of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201 (J.R.L., I.G., Z.G., L.T., J.D.D.); and Medical Biotechnology Center and Department of Chemical and Biochemical Engineering, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201 (G.R.).

Govind RaoDepartment of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, Maryland 21201 (J.R.L., I.G., Z.G., L.T., J.D.D.); and Medical Biotechnology Center and Department of Chemical and Biochemical Engineering, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201 (G.R.).

Funding

WHO'S WHO IN FLUORESCENCEP41RR008119 · NCRR · UNIVERSITY OF MARYLAND BALTIMORE · PI BASKAKOV, ILIA V · 1993 to 2006

$7.4M

NCRR NIH HHS P41 RR008119

6 · The paper itself

Abstract

We describe a new method of fluorescence sensing based on fluorescence polarization. The sensor consists of two compartments, both of which contain the sensing fluorophore. One side of the sensor contains a constant concentration of analyte, and the other contains the unknown concentration. Emission from both sides is observed through polarizers, with the polarization from the sample being rotated 90° from that of the reference. Changes in the fluorescence intensity of the sample result in changes in the measured polarization for the combined emission. We show that this approach can be used to measure glucose and calcium using fluorophores which show analyte-dependent intensity changes, and no change in the spectral shape. Only a single fluorophore is required, this being the sensing fluorophore in both sides of the sensor. We also show that polarization sensing of glucose and calcium can be performed with visual detection of the polarization. In this case the only electronic component is the light source. These simple schemes can be used with a variety of analytes. The only requirement is a change in fluorescence intensity in response to the analyte.

Indexed as

AnistropyCalciumFluorescence sensingGlucosePolarizationProtein sensors

Identifiers

PMID32255815

DOI10.1366/0003702991947964

PMCPMC7133461

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.