Evidence map›Paper›PMID 32253985›Full record

ArticleStem cells and development2020

Normothermic Machine Perfusion Combined with Bone Marrow Mesenchymal Stem Cells Improves the Oxidative Stress Response and Mitochondrial Function in Rat Donation After Circulatory Death Livers.

Liu Yang, Huan Cao, Dong Sun, Ling Lin, Wei-Ping Zheng, Zhong-Yang Shen, Hong-Li Song

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells and development, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
5.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 38 citations in OpenAlex.

  1. Pooled it
  2. Small animalWorld journal of gastroenterology · 2026
    Review
  3. Review
  4. Review
  5. Article
  6. Review
  7. Review
  8. Review
  9. Machine Perfusion and Bioengineering Strategies in Transplantation-Beyond the Emerging Concepts.Transplant international : official journal of the European Society for Organ Transplantation · 2024
    Review
  10. Article
  11. Review
  12. Article
  13. Organ Repair and Regeneration DuringTransplant international : official journal of the European Society for Organ Transplantation · 2023
    Review
  14. Review
  15. The current status of stem cell-based therapies during ex vivo graft perfusion: An integrated review of four organs.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2022
    Review
  16. Review
  17. Article
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Liu YangTianjin First Central Hospital Clinic Institute, Tianjin Medical University, Tianjin, People's Republic of China.
Huan CaoTianjin First Central Hospital Clinic Institute, Tianjin Medical University, Tianjin, People's Republic of China.
Dong SunTianjin First Central Hospital Clinic Institute, Tianjin Medical University, Tianjin, People's Republic of China.
Ling LinTianjin First Central Hospital Clinic Institute, Tianjin Medical University, Tianjin, People's Republic of China.
Wei-Ping ZhengDepartment of Organ Transplantation, Tianjin First Central Hospital, Tianjin, People's Republic of China.
Zhong-Yang ShenDepartment of Organ Transplantation, Tianjin First Central Hospital, Tianjin, People's Republic of China.
Hong-Li SongDepartment of Organ Transplantation, Tianjin First Central Hospital, Tianjin, People's Republic of China.
Tianjin First Center Hospital · CNChinese Academy of Medical Sciences & Peking Union Medical College · CNTianjin University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

There is a need to improve the quality of donor liver from donation after circulatory death (DCD). The purpose of this study was to investigate the effects and mechanism of normothermic machine perfusion (NMP) combined with bone marrow mesenchymal stem cells (BMMSCs) on the oxidative stress and mitochondrial function in DCD livers. DCD livers were obtained, a rat NMP system was established, and BMMSCs were extracted and identified. The DCD livers were grouped by their preservation method: Normal, static cold storage (SCS), NMP (P), and NMP combined with BMMSCs (PB), and the preservation time was up to 8 h. An IAR20 cell oxidative stress injury model was established in vitro by simulating DCD oxidative stress injury and coculturing with BMMSCs for 6 h. Compared with SCS group, after 6 h in vitro, the PB and P groups had significantly improved liver function and liver histological damage, reduced hepatocyte apoptosis and oxidative stress, improved hepatocyte mitochondrial damage, and increased mitochondrial membrane potential. These indicators were significantly better in the PB group than in the P group. BMMSCs significantly inhibited reactive oxygen species release from the IAR20 cell oxidative stress model in vitro, ameliorated mitochondrial damage, and increased mitochondrial membrane potential level. BMMSCs also downregulated the JUN N-terminal kinase-nuclear factor kappa B (JNK-NF-κB) signaling pathway significantly in the IAR20 cell oxidative stress model and promoted AMP-activated protein kinase (AMPK) activation. We verified that NMP combined with BMMSCs also played the same role in the PB group. NMP combined with BMMSCs could improve liver quality by relieving oxidative stress injury and improving mitochondrial function in rat DCD livers. The mechanism of protective role might involve inhibiting the JNK-NF-κB pathway to reduce oxidative stress and promote AMPK activation, thereby reducing mitochondrial damage and increase mitochondrial function.

Indexed as

Oxidative StressAllograftsAMP-Activated Protein Kinase KinasesAnimalsCells, CulturedInfusion PumpsIschemiaJanus KinasesLiverLiver TransplantationMaleMembrane Potential, MitochondrialMesenchymal Stem Cell TransplantationMitochondria, LiverNF-kappa BOrgan PreservationAMP-Activated Protein Kinase KinasesJanus KinasesNF-kappa BProtein KinasesAMP-activated protein kinasebone marrow mesenchymal stem cellsJNK-NF-κB signaling pathwaymitochondrianormothermic machine perfusion

Identifiers

PMID32253985
PMCPMC7336881
OpenAlexW3015006527

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.