ArticleParasitology research2020
Germ cells: a useful tool for the taxonomy of Rhipicephalus sanguineus s.l. and species of the Amblyomma cajennense complex (Acari: Ixodidae).
Article in Parasitology research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed, 1 synthesis or guideline pooled it, 4 citations in OpenAlex.
- Ticks (Acari: Ixodoidea) associated with mammals in Colombia: a historical review, molecular species confirmation, and establishment of new relationships.Parasitology research · 2021Pooled it
- Infestation, histology, and molecular confirmation ofInternational journal for parasitology. Parasites and wildlife · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The systematics of several ticks species (Acari: Ixodidae) remains controversial. Many species, including those of the Amblyomma cajennense complex and Rhipicephalus sanguineus s.l., are given special attention since they are cryptic species complexes and are also important in human and veterinary medicine. The A. cajennense complex was recently reorganized into six valid species, among which Amblyomma patinoi and Amblyomma mixtum have been confirmed in Colombia. On the other hand, the taxonomic status of R. sanguineus s.l. is controversial since it is a cosmopolitan cryptic species complex with a high reproductive capacity and a broad range of hosts (including man). To address this challenge, the germ cells of male ticks display a diverse morphology that offers novel opportunities for taxonomy. This study describes the events of spermatogenesis in A. mixtum and R. sanguineus s.l. individuals collected during active feeding on domestic hosts in the department of Caldas, Colombia. The individuals were identified using dichotomous keys and through PCR amplification of a fragment of the mitochondrial 16S ribosomal DNA gene. The male reproductive systems of A. mixtum and R. sanguineus s.l. were fixed in 2.5% glutaraldehyde for 48 h and dehydrated in increasing dilutions of ethanol. The samples were then embedded and mounted in historesin to obtain sections of 3 μm that were stained with hematoxylin-eosin (HE), photographed, and visualized through optical microscopy. The results show that the morphology of mature germ cells displays excellent diagnostic traits that can be used for tick taxonomy.
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Registered trials
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