ArticlePloS one2020
Effects of sex and chronic cigarette smoke exposure on the mouse cecal microbiome.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Longitudinal dynamics and site-specific recovery of the human respiratory microbiome following smoking cessation.Respiratory research · 2026Article
- Longitudinal multi-omics evidence reveals lung injury and concurrent disruption of intestinal flora and serum metabolism by cigarette smoke and influenza virus.Frontiers in cellular and infection microbiology · 2026Article
- Smoking-induced microbial dysbiosis: a key driver of systemic diseases and emerging therapeutic opportunities.Cell death discovery · 2025Review
- The influence of environment on bacterial co-abundance in the gut microbiomes of healthy human individuals.Communications biology · 2025Article
- Gut Microbiota at the Crossroad of Hepatic Oxidative Stress and MASLD.Antioxidants (Basel, Switzerland) · 2025Review
- Integrative network pharmacology, transcriptomics, and microbiomics elucidate the therapeutic mechanism ofFrontiers in microbiology · 2025Article
- Chronic Exposure to Both Electronic and Conventional Cigarettes Alters Ileum and Colon Turnover, Immune Function, and Barrier Integrity in Mice.Journal of xenobiotics · 2024Article
- When smoke meets gut: deciphering the interactions between tobacco smoking and gut microbiota in disease development.Science China. Life sciences · 2024Review
- Lifestyle patterns influence the composition of the gut microbiome in a healthy Chinese population.Scientific reports · 2023Article
- Tobacco smoke exacerbates Filifactor alocis pathogenicity.Journal of clinical periodontology · 2023Article
- Smoking-induced microbial dysbiosis in health and disease.Clinical science (London, England : 1979) · 2022Review
- Effects of Cigarette Smoke Exposure on the Gut Microbiota and Liver Transcriptome in Mice Reveal Gut-Liver Interactions.International journal of molecular sciences · 2022Article
- Taxonomic composition and variation in the gut microbiota of laboratory mice.Mammalian genome : official journal of the International Mammalian Genome Society · 2021Review
- Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice.Environmental health perspectives · 2021Article
- Active Smoking Induces Aberrations in Digestive Tract Microbiota of Rats.Frontiers in cellular and infection microbiology · 2021Article
- Impact of 6-Month Exposure to Aerosols From Potential Modified Risk Tobacco Products Relative to Cigarette Smoke on the Rodent Gastrointestinal Tract.Frontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
rationaleChronic smoke exposure is associated with weight loss in patients with Chronic Obstructive Pulmonary Disease (COPD). However, the biological contribution of chronic smoking and sex on the cecal microbiome has not been previously investigated.
methodsAdult male, female and ovariectomized mice were exposed to air (control group) or smoke for six months using a standard nose-only smoke exposure system. DNA was extracted from the cecal content using the QIAGEN QIAamp® DNA Mini Kit. Droplet digital PCR was used to generate total 16S bacterial counts, followed by Illumina MiSeq® analysis to determine microbial community composition. The sequencing data were resolved into Amplicon Sequence Variants and analyzed with the use of QIIME2®. Alpha diversity measures (Richness, Shannon Index, Evenness and Faith's Phylogenetic Diversity) and beta diversity (based on Bray-Curtis distances) were assessed and compared according to smoke exposure and sex.
resultsThe microbial community was different between male and female mice, while ovariectomy made the cecal microbiome similar to that of male mice. Chronic smoke exposure led to significant changes in the cecal microbial community in both male and female mice. The organism, Alistipes, was the most consistent bacteria identified at the genus level in the cecal content that was reduced with chronic cigarette exposure and its expression was positively related to the whole-body weight of these mice.
conclusionChronic smoke exposure is associated with changes in the cecal content microbiome; these changes may play a role in the weight changes that are observed in cigarette smokers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.