ArticleNature methods2020
Genetic tool development in marine protists: emerging model organisms for experimental cell biology.
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Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 69 papers.
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69citing papers in PubMed
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Who cites it
69 citing papers in PubMed.
- Biological and genomic resources for the cosmopolitan phytoplankton Bathycoccus: insights into genetic diversity and function of outlier chromosomes.The Plant journal : for cell and molecular biology · 2026Article
- Unlocking CRISPR-Cas9 editing for widely diverse Dictyostelid species.Molecular systems biology · 2026Article
- Marine Bioactive Compounds from Functional Seafoods: Pharmacological Mechanisms and Health Applications.Marine drugs · 2026Review
- Origin of replication discovery for environmentally isolatediScience · 2026Article
- Transgenic approaches in the pathogenic free-living amoebae: what are the hurdles?Parasitology · 2026Review
- Genetic Manipulation of Paradiplonema papillatum.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Development of Approaches for Transgene Expression in the Pathogenic Free-Living AmoebaPathogens (Basel, Switzerland) · 2025Article
- Evolutionary cell biology comes of age.Journal of cell science · 2025Article
- Subcellular proteomics of the protist Paradiplonema papillatum reveals the digestive capacity of the cell membrane and the plasticity of peroxisomes across euglenozoans.PLoS biology · 2025Article
- A genetic transformation system for the heterotrophic diatom Nitzschia putrida (Bacillariophyceae).Journal of phycology · 2025Article
- More realistic plankton simulation models will improve projections of ocean ecosystem responses to global change.Nature ecology & evolution · 2025Review
- Physiology and metabolism of eukaryotic microalgae involved in aquatic photosymbioses.The New phytologist · 2025Review
- Prevalence and environmental abundance of the TSET complex in cosmopolitan algal groups.iScience · 2025Article
- A game of tag: A review of protein tags for the successful detection, purification and fluorescence labelling of proteins expressed in microalgae.The Plant journal : for cell and molecular biology · 2025Review
- Converting Blastocrithidia Nonstop, a Trypanosomatid With Non-Canonical Genetic Code, Into a Genetically-Tractable Model.Molecular microbiology · 2025Article
- The fate of artificial transgenes in Acanthamoeba castellanii.BMC genomics · 2025Article
- Development of Antisense Tools to Study Bodo saltans and Its Intracellular Symbiont.MicrobiologyOpen · 2025Article
- Article
- Genetic engineering in diatoms: advances and prospects.The Plant journal : for cell and molecular biology · 2025Review
- Half of microbial eukaryote literature focuses on only 12 human parasites.The ISME journal · 2025Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
113 authors.
Drahomíra Faktorová *Institute of Parasitology, Biology Centre, Czech Academy of Sciences and Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic. dranov@paru.cas.cz.ORCID http://orcid.org/0000-0001-9623-2233
R Ellen R Nisbet *Department of Biochemistry, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0003-4487-196X
José A Fernández Robledo *Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, USA.
Elena Casacuberta *Institut de Biologia Evolutiva, CSIC-Universitat Pompeu Fabra, Barcelona, Spain.
Lisa Sudek *Monterey Bay Aquarium Research Institute, Moss Landing, CA, USA.
Andrew E AllenIntegrative Oceanography Division, Scripps Institution of Oceanography, University of California, San Diego, CA, USA.ORCID http://orcid.org/0000-0001-5911-6081
Manuel AresMolecular, Cell and Developmental Biology, University of California, Santa Cruz, CA, USA.ORCID http://orcid.org/0000-0002-2552-9168
Cristina ArestéInstitut de Biologia Evolutiva, CSIC-Universitat Pompeu Fabra, Barcelona, Spain.
Cecilia BalestreriThe Marine Biological Association, Plymouth and School of Ocean and Earth Sciences, University of Southampton, Southampton, UK.
Adrian C BarbrookDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Patrick BeardsleeSchool of Biological Sciences, University of Nebraska, Lincoln, NE, USA.
Sara BenderGordon and Betty Moore Foundation, Palo Alto, CA, USA.
David S BoothDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
François-Yves BougetSorbonne Université, CNRS UMR7621, Observatoire Océanologique, Banyuls sur Mer, France.
Chris BowlerInstitut de Biologie de l'Ecole Normale Supérieure (IBENS), Ecole Normale Supérieure, CNRS, INSERM, Université PSL, Paris, France.ORCID http://orcid.org/0000-0003-3835-6187
Susana A BregliaCentre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, Nova Scotia, Canada.
Colin BrownleeThe Marine Biological Association, Plymouth and School of Ocean and Earth Sciences, University of Southampton, Southampton, UK.
Gertraud BurgerDepartment of Biochemistry and Robert-Cedergren Centre for Bioinformatics and Genomics, Université de Montréal, Montreal, Quebec, Canada.ORCID http://orcid.org/0000-0002-8679-8812
Heriberto CeruttiSchool of Biological Sciences, University of Nebraska, Lincoln, NE, USA.
Rachele CesaroniInstitute of Cell Biology, University of Bern, Bern, Switzerland.
Miguel A ChiurilloCenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, USA.
Thomas ClementeSchool of Biological Sciences, University of Nebraska, Lincoln, NE, USA.
Duncan B ColesBigelow Laboratory for Ocean Sciences, East Boothbay, ME, USA.
Jackie L CollierSchool of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY, USA.ORCID http://orcid.org/0000-0001-8774-5715
Elizabeth C CooneyDepartment of Botany, University of British Columbia, Vancouver, British Columbia, Canada.ORCID http://orcid.org/0000-0001-7435-7609
Kathryn CoyneUniversity of Delaware College of Earth, Ocean and Environment, Lewes, DE, USA.
Roberto DocampoCenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, USA.ORCID http://orcid.org/0000-0003-4229-8784
Christopher L DupontMicrobial and Environmental Genomics, J. Craig Venter Institute, La Jolla, CA, USA.ORCID http://orcid.org/0000-0002-0896-6542
Virginia EdgcombWoods Hole Oceanographic Institution, Woods Hole, MA, USA.ORCID http://orcid.org/0000-0001-6805-381X
Elin EinarssonDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Pía A ElustondoCentre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, Nova Scotia, Canada.
Fernan FedericiFacultad Ciencias Biológicas, Pontificia Universidad Católica de Chile, Fondo de Desarrollo de Areas Prioritarias, Center for Genome Regulation and Millennium Institute for Integrative Biology (iBio), Santiago de Chile, Chile.
Veronica Freire-BeneitezSchool of Biosciences, University of Kent, Canterbury, Kent, UK.
Nastasia J FreyriaBigelow Laboratory for Ocean Sciences, East Boothbay, ME, USA.ORCID http://orcid.org/0000-0002-4972-9383
Kodai FukudaGraduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan.
Paulo A GarcíaDepartment of Mechanical Engineering, Massachusetts Institute of Technology, Boston, MA, USA.
Peter R GirguisDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.
Fatma GomaaDepartment of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.
Sebastian G GornikCentre for Organismal Studies, University of Heidelberg, Heidelberg, Germany.ORCID http://orcid.org/0000-0002-8026-1336
Jian GuoMonterey Bay Aquarium Research Institute, Moss Landing, CA, USA.
Vladimír HamplDepartment of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic.
Yutaka HanawaFaculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan.
Esteban R Haro-ContrerasCentre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, Nova Scotia, Canada.
Elisabeth HehenbergerDepartment of Botany, University of British Columbia, Vancouver, British Columbia, Canada.
Andrea HighfieldThe Marine Biological Association, Plymouth and School of Ocean and Earth Sciences, University of Southampton, Southampton, UK.
Yoshihisa HirakawaFaculty of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan.
Amanda HopesSchool of Environmental Sciences, University of East Anglia, Norwich, UK.
Christopher J HoweDepartment of Biochemistry, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0002-6975-8640
Ian HuDepartment of Biochemistry, University of Cambridge, Cambridge, UK.ORCID http://orcid.org/0000-0002-2287-031X
Jorge IbañezFacultad Ciencias Biológicas, Pontificia Universidad Católica de Chile, Fondo de Desarrollo de Areas Prioritarias, Center for Genome Regulation and Millennium Institute for Integrative Biology (iBio), Santiago de Chile, Chile.
Nicholas A T IrwinDepartment of Botany, University of British Columbia, Vancouver, British Columbia, Canada.
Yuu IshiiGraduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan.ORCID http://orcid.org/0000-0003-1735-9557
Natalia Ewa JanowiczDepartment of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic.
Adam C JonesGordon and Betty Moore Foundation, Palo Alto, CA, USA.ORCID http://orcid.org/0000-0001-7521-1863
Ambar KachaleInstitute of Parasitology, Biology Centre, Czech Academy of Sciences and Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic.
Konomi Fujimura-KamadaDivision of Environmental Photobiology, National Institute for Basic Biology, Okazaki, Aichi, Japan.
Binnypreet KaurInstitute of Parasitology, Biology Centre, Czech Academy of Sciences and Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic.
Jonathan Z KayeGordon and Betty Moore Foundation, Palo Alto, CA, USA.
Eleanna KazanaSchool of Biosciences, University of Kent, Canterbury, Kent, UK.
Patrick J KeelingDepartment of Botany, University of British Columbia, Vancouver, British Columbia, Canada.
Nicole KingDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Lawrence A KlobutcherDepartment of Marine Sciences, University of Connecticut , Groton, CT, USA.
Noelia LanderCenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, USA.
Imen LassadiDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Zhuhong LiCenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA, USA.
Senjie LinDepartment of Marine Sciences, University of Connecticut , Groton, CT, USA.ORCID http://orcid.org/0000-0001-8831-6111
Jean-Claude LozanoSorbonne Université, CNRS UMR7621, Observatoire Océanologique, Banyuls sur Mer, France.
Fulei LuanSchool of Biological Sciences, University of Nebraska, Lincoln, NE, USA.
Shinichiro MaruyamaGraduate School of Life Sciences, Tohoku University, Sendai, Miyagi, Japan.ORCID http://orcid.org/0000-0002-1128-5916
Tamara MatuteFacultad Ciencias Biológicas, Pontificia Universidad Católica de Chile, Fondo de Desarrollo de Areas Prioritarias, Center for Genome Regulation and Millennium Institute for Integrative Biology (iBio), Santiago de Chile, Chile.
Cristina MiceliSchool of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Jun MinagawaDivision of Environmental Photobiology, National Institute for Basic Biology, Okazaki, Aichi, Japan.ORCID http://orcid.org/0000-0002-3028-3203
Mark MoosburnerIntegrative Oceanography Division, Scripps Institution of Oceanography, University of California, San Diego, CA, USA.
Sebastián R NajleInstitut de Biologia Evolutiva, CSIC-Universitat Pompeu Fabra, Barcelona, Spain.ORCID http://orcid.org/0000-0002-0654-9147
Deepak NanjappaUniversity of Delaware College of Earth, Ocean and Environment, Lewes, DE, USA.
Isabel C NimmoDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Luke NobleCenter for Genomics and Systems Biology, New York University, New York, NY, USA.
Anna M G Novák VanclováDepartment of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic.
Mariusz NowackiInstitute of Cell Biology, University of Bern, Bern, Switzerland.ORCID http://orcid.org/0000-0003-4894-2905
Isaac NuñezFacultad Ciencias Biológicas, Pontificia Universidad Católica de Chile, Fondo de Desarrollo de Areas Prioritarias, Center for Genome Regulation and Millennium Institute for Integrative Biology (iBio), Santiago de Chile, Chile.
Arnab PainBiological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.ORCID http://orcid.org/0000-0002-1755-2819
Angela PiersantiSchool of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Sandra PucciarelliSchool of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.
Jan PyrihSchool of Biosciences, University of Kent, Canterbury, Kent, UK.
Joshua S RestDepartment of Ecology and Evolution, Stony Brook University, Stony Brook, NY, USA.
Mariana RiusSchool of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY, USA.
Deborah RobertsonLasry Center for Biosciences, Clark University, Worcester, MA, USA.
Albane RuaudFacultad Ciencias Biológicas, Pontificia Universidad Católica de Chile, Fondo de Desarrollo de Areas Prioritarias, Center for Genome Regulation and Millennium Institute for Integrative Biology (iBio), Santiago de Chile, Chile.
Iñaki Ruiz-TrilloInstitut de Biologia Evolutiva, CSIC-Universitat Pompeu Fabra, Barcelona, Spain.
Monika A SiggDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Pamela A SilverDepartment of Systems Biology, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0002-7856-4071
Claudio H SlamovitsCentre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, Nova Scotia, Canada.
G Jason SmithDepartment of Environmental Biotechnology, Moss Landing Marine Laboratories, Moss Landing, CA, USA.ORCID http://orcid.org/0000-0003-1258-4800
Brittany N SprecherDepartment of Marine Sciences, University of Connecticut , Groton, CT, USA.ORCID http://orcid.org/0000-0001-5032-3834
Rowena SternThe Marine Biological Association, Plymouth and School of Ocean and Earth Sciences, University of Southampton, Southampton, UK.
Estienne C SwartInstitute of Cell Biology, University of Bern, Bern, Switzerland.
Anastasios D TsaousisSchool of Biosciences, University of Kent, Canterbury, Kent, UK.ORCID http://orcid.org/0000-0002-5424-1905
Lev TsypinDepartment of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA.ORCID http://orcid.org/0000-0002-0642-8468
Aaron TurkewitzDepartment of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, USA.ORCID http://orcid.org/0000-0003-3531-5806
Jernej TurnšekIntegrative Oceanography Division, Scripps Institution of Oceanography, University of California, San Diego, CA, USA.ORCID http://orcid.org/0000-0002-9056-3565
Matus ValachDepartment of Biochemistry and Robert-Cedergren Centre for Bioinformatics and Genomics, Université de Montréal, Montreal, Quebec, Canada.ORCID http://orcid.org/0000-0001-8689-0080
Valérie VergéSorbonne Université, CNRS UMR7621, Observatoire Océanologique, Banyuls sur Mer, France.
Peter von DassowFacultad Ciencias Biológicas, Pontificia Universidad Católica de Chile, Fondo de Desarrollo de Areas Prioritarias, Center for Genome Regulation and Millennium Institute for Integrative Biology (iBio), Santiago de Chile, Chile.
Tobias von der HaarSchool of Biosciences, University of Kent, Canterbury, Kent, UK.ORCID http://orcid.org/0000-0002-6031-9254
Ross F WallerDepartment of Biochemistry, University of Cambridge, Cambridge, UK.
Lu WangInstitute of Oceanography, Minjiang University, Fuzhou, China.
Xiaoxue WenSchool of Biological Sciences, University of Nebraska, Lincoln, NE, USA.
Glen WheelerThe Marine Biological Association, Plymouth and School of Ocean and Earth Sciences, University of Southampton, Southampton, UK.
April WoodsDepartment of Environmental Biotechnology, Moss Landing Marine Laboratories, Moss Landing, CA, USA.
Huan ZhangDepartment of Marine Sciences, University of Connecticut , Groton, CT, USA.
Thomas MockSchool of Environmental Sciences, University of East Anglia, Norwich, UK. t.mock@uea.ac.uk.ORCID http://orcid.org/0000-0001-9604-0362
Alexandra Z WordenMonterey Bay Aquarium Research Institute, Moss Landing, CA, USA. azworden@geomar.de.ORCID http://orcid.org/0000-0002-9888-9324
Julius LukešInstitute of Parasitology, Biology Centre, Czech Academy of Sciences and Faculty of Sciences, University of South Bohemia, České Budějovice, Czech Republic. jula@paru.cas.cz.ORCID http://orcid.org/0000-0002-0578-6618
Funding
STRUCTURE/FUNCTION OF YEAST SMALL NUCLEAR RNPSR01GM040478 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI ARES, MANUEL · 1988 to 2021
$10.8MNIGMS NIH HHS R01 GM040478
Abstract
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.
Indexed as
Marine BiologyModels, BiologicalTransformation, GeneticBiodiversityDNAEcosystemEnvironmentEukaryotaGreen Fluorescent ProteinsSpecies SpecificityDNAGreen Fluorescent Proteins
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