ArticleScientific reports2020
Blast resistance gene Pi54 over-expressed in rice to understand its cellular and sub-cellular localization and response to different pathogens.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 36 citations in OpenAlex.
- Development of dual disease resistant Basmati rice varieties offer significant economic advantage and impetus to sustainable crop production.Journal of advanced research · 2025Article
- Deciphering the Contribution of TATA Box and 5'UTR to Defense Signaling in Rice Under Blast Infection.Biology · 2025Review
- Improving the cultivated rice Sakha104 (PeerJ · 2025Article
- Strong culm: a crucial trait for developing next-generation climate-resilient rice lines.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Article
- Emergence of Rice BlastLife (Basel, Switzerland) · 2023Article
- POLLEN WALL ABORTION 1 is essential for pollen wall development in rice.Plant physiology · 2022Article
- Understanding the Dynamics of Blast Resistance in Rice-Journal of fungi (Basel, Switzerland) · 2022Review
- Closer vein spacing by ectopic expression of nucleotide-binding and leucine-rich repeat proteins in rice leaves.Plant cell reports · 2022Article
- Computational models for prediction of protein-protein interaction in rice andFrontiers in plant science · 2022Article
- Deciphering the role of microRNAs duringPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
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Authors and funding
6 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Rice blast resistance gene, Pi54 provides broad-spectrum resistance against different strains of Magnaporthe oryzae. Understanding the cellular localization of Pi54 protein is an essential step towards deciphering its place of interaction with the cognate Avr-gene. In this study, we investigated the sub-cellular localization of Pi54 with Green Fluorescent Protein (GFP) as a molecular tag through transient and stable expression in onion epidermal cells (Allium cepa) and susceptible japonica cultivar rice Taipei 309 (TP309), respectively. Confocal microscopy based observations of the onion epidermal cells revealed nucleus and cytoplasm specific GFP signals. In the stable transformed rice plants, GFP signal was recorded in the stomata, upper epidermal cells, mesophyll cells, vascular bundle, and walls of bundle sheath and bulliform cells of leaf tissues. These observations were further confirmed by Immunocytochemical studies. Using GFP specific antibodies, it was found that there was sufficient aggregation of GFP::Pi54protein in the cytoplasm of the leaf mesophyll cells and periphery of the epidermal cells. Interestingly, the transgenic lines developed in this study could show a moderate level of resistance to Xanthomonas oryzae and Rhizoctonia solani, the causal agents of the rice bacterial blight and sheath blight diseases, respectively. This study is a first detailed report, which emphasizes the cellular and subcellular distribution of the broad spectrum blast resistance gene Pi54 in rice and the impact of its constitutive expression towards resistance against other fungal and bacterial pathogens of rice.
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