Evidence map›Paper›PMID 32250601›Full record

ArticleAnalytical chemistry2020

Benchmarking the Orbitrap Tribrid Eclipse for Next Generation Multiplexed Proteomics.

Qing Yu, Joao A Paulo, Jose Naverrete-Perea, Graeme C McAlister, Jesse D Canterbury, Derek J Bailey, Aaron M Robitaille, Romain Huguet, Vlad Zabrouskov, Steven P Gygi and 1 more

Abstract read
In one paragraph

Article in Analytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed.

  1. Article
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  15. Technologies to Study Genetics and Molecular Pathways.Advances in experimental medicine and biology · 2024
    Review
  16. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Qing YuHarvard Medical School, Boston, Massachusetts 02115, United States.ORCID 0000-0003-0468-5353
Joao A PauloHarvard Medical School, Boston, Massachusetts 02115, United States.
Jose Naverrete-PereaHarvard Medical School, Boston, Massachusetts 02115, United States.ORCID 0000-0001-6265-6214
Graeme C McAlisterThermo Fisher Scientific, San Jose, California 95134, United States.
Jesse D CanterburyThermo Fisher Scientific, San Jose, California 95134, United States.
Derek J BaileyThermo Fisher Scientific, San Jose, California 95134, United States.
Aaron M RobitailleThermo Fisher Scientific, San Jose, California 95134, United States.
Romain HuguetThermo Fisher Scientific, San Jose, California 95134, United States.
Vlad ZabrouskovThermo Fisher Scientific, San Jose, California 95134, United States.
Steven P GygiHarvard Medical School, Boston, Massachusetts 02115, United States.ORCID 0000-0001-7626-0034
Devin K SchweppeHarvard Medical School, Boston, Massachusetts 02115, United States.ORCID 0000-0002-3241-6276

Funding

New Sample Multiplexing Technologies to Identify Chemical Probes and Illuminate Ubiquitin BiologyR01GM067945 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI GYGI, STEVEN P · 2003 to 2024
$10.5M
Advancing Multiplexed Isobaric Tag-based Strategies for Proteome ProfilingR01GM132129 · NIGMS · HARVARD MEDICAL SCHOOL · PI PAULO, JOAO A · 2019 to 2023
$1.7M
NIGMS NIH HHS R01 GM067945NIGMS NIH HHS R01 GM132129
6 · The paper itself

Abstract

The rise of sample multiplexing in quantitative proteomics for the dissection of complex phenotypic comparisons has been advanced by the development of ever more sensitive and robust instrumentation. Here, we evaluated the utility of the Orbitrap Eclipse Tribrid mass spectrometer (advanced quadrupole filter, optimized FTMS scan overhead) and new instrument control software features (Precursor Fit filtering, TurboTMT and Real-time Peptide Search filtering). Multidimensional comparisons of these novel features increased total peptide identifications by 20% for SPS-MS

Indexed as

ProteomicsMass SpectrometryPeptidesPeptides

Identifiers

PMID32250601
PMCPMC7295122

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.