ArticleeLife2020
Zebrafish embryonic explants undergo genetically encoded self-assembly.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed.
- Article
- Tissue rigidity phase transition shapes morphogen gradients.Nature cell biology · 2026Article
- Adhesion-driven rigidity transition decoupled from density-driven jamming triggers epithelial organization in embryonic tissues.Nature physics · 2026Article
- Live imaging and functional characterization of the avian hypoblast redefine the mechanisms of primitive streak induction.Nature communications · 2025Article
- Temporal dynamics of BMP/Nodal ratio drive tissue-specific gastrulation morphogenesis.Development (Cambridge, England) · 2025Article
- Established and emerging new approach methodologies in neuroscience.Frontiers in neuroscience · 2025Review
- Early autonomous patterning of the anteroposterior axis in gastruloids.Development (Cambridge, England) · 2024Article
- The logic of monsters: development and morphological diversity in stem-cell-based embryo models.Interface focus · 2024Review
- Axis formation in annual killifish: Nodal and β-catenin regulate morphogenesis without Huluwa prepatterning.Science (New York, N.Y.) · 2024Article
- Robust axis elongation by Nodal-dependent restriction of BMP signaling.Development (Cambridge, England) · 2024Article
- Temporal dynamics of BMP/Nodal ratio drive tissue-specific gastrulation morphogenesis.bioRxiv : the preprint server for biology · 2024Article
- Live Imaging Transverse Sections of Zebrafish Embryo Explants.Bio-protocol · 2024Article
- Protocol for extracting live blastoderm cells from embryos of annual killifish.STAR protocols · 2023Article
- A Yap-dependent mechanoregulatory program sustains cell migration for embryo axis assembly.Nature communications · 2023Article
- Article
- Review
- Origin, form and function of extraembryonic structures in teleost fishes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Ethanol Effects on Early Developmental Stages Studied Using the Zebrafish.Biomedicines · 2022Review
- Stem-cell-based human and mouse embryo models.Current opinion in genetics & development · 2022Review
- The unappreciated generative role of cell movements in pattern formation.Royal Society open science · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Embryonic stem cell cultures are thought to self-organize into embryoid bodies, able to undergo symmetry-breaking, germ layer specification and even morphogenesis. Yet, it is unclear how to reconcile this remarkable self-organization capacity with classical experiments demonstrating key roles for extrinsic biases by maternal factors and/or extraembryonic tissues in embryogenesis. Here, we show that zebrafish embryonic tissue explants, prepared prior to germ layer induction and lacking extraembryonic tissues, can specify all germ layers and form a seemingly complete mesendoderm anlage. Importantly, explant organization requires polarized inheritance of maternal factors from dorsal-marginal regions of the blastoderm. Moreover, induction of endoderm and head-mesoderm, which require peak Nodal-signaling levels, is highly variable in explants, reminiscent of embryos with reduced Nodal signals from the extraembryonic tissues. Together, these data suggest that zebrafish explants do not undergo
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.