Evidence map›Paper›PMID 32248380›Full record

ArticleMikrochimica acta2020

A fluorescent immunosensor for determination and imaging of circulating tumor cells based on a bifunctional DNA nanomachine.

Ye Zhang, Shihua Luo, Bo Situ, Xinyi Ye, Yifang Huang, Bo Li, Xiujuan Jiang, Xueping Chen, Lei Zheng, Xiaohui Yan

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Ye ZhangDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, Guangdong Province, People's Republic of China.
Shihua LuoDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, Guangdong Province, People's Republic of China.
Bo SituDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, Guangdong Province, People's Republic of China.
Xinyi YeDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, Guangdong Province, People's Republic of China.
Yifang HuangDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, Guangdong Province, People's Republic of China.
Bo LiDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, Guangdong Province, People's Republic of China.
Xiujuan JiangDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, Guangdong Province, People's Republic of China.
Xueping ChenThe Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical University, 400016, Chongqing, People's Republic of China.
Lei ZhengDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, Guangdong Province, People's Republic of China. nfyyzhenglei@smu.edu.cn.ORCID http://orcid.org/0000-0003-2576-8780
Xiaohui YanDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical University, 510515, Guangzhou, Guangdong Province, People's Republic of China. gzyanxh@126.com.
Nanfang Hospital · CNThe Affiliated Yongchuan Hospital of Chongqing Medical University · CN

Funding

National Natural Science Foundation of China 81802114
6 · The paper itself

Abstract

A fluorescent platform was developed for the determination and visualization of circulating tumor cells by a toehold-mediated bifunctional DNA nanomachine. In the presence of target tumor cells, the DNA nanomachine was activated. Multiple DNA products were formed, including dendritic DNA products and double-strand DNA products. Dendritic DNA products bound to their target cells for the visualization, while double-strand DNA products were released for the determination of tumor cells. At fluorescence excitation and emission wavelengths of 530 and 550 nm, this method could detect as low as 43 cells/mL (S/N = 3) with a linear range of 100 to 10,000 cells/mL. In clinical hydrothorax samples, this platform exhibited high reliability with a recovery of 93 to 116%. At the fluorescence excitation and emission wavelengths of 490 and 515 nm, the specificity and biocompatibility of this method were further verified by tumor cells imaging. Furthermore, the robustness of the toehold-mediated bifunctional DNA nanomachine was demonstrated by the specific gene mutation detection in single-cell analysis. Graphical abstract Schematic illustration of the fluorescent immunosensor for determination and imaging of circulating tumor cells. The method is based on aptamer-based recognition and toehold-mediated bifunctional DNA nanomachine.

Indexed as

Neoplastic Cells, CirculatingAptamers, NucleotideBiosensing TechniquesCell Line, TumorDNADNA ProbesFluorescent DyesHumansHydrothoraxLimit of DetectionMicroscopy, ConfocalMicroscopy, FluorescenceNucleic Acid HybridizationNucleolinPhosphoproteinsRNA-Binding ProteinsAptamers, NucleotideDNADNA ProbesFluorescent DyesNucleolinPhosphoproteinsRNA-Binding ProteinsFluorescent platformGene mutation detectionToehold-mediatedVisualization

Identifiers

PMID32248380
OpenAlexW3014726782

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.