Evidence map›Paper›PMID 32248284›Full record

ArticleCurrent microbiology2020

Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity.

Sujan Khadka, Sanjib Adhikari, Alina Thapa, Raju Panday, Manjila Adhikari, Sanjeep Sapkota, Ramesh Sharma Regmi, Namita Paudel Adhikari, Ram Proshad, Niranjan Koirala

Abstract read
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In one paragraph

Article in Current microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.3field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Journal of fungi (Basel, Switzerland) · 2022
    Review
  6. Plants (Basel, Switzerland) · 2022
    Article
  7. Detection ofScientifica · 2022
    Article
  8. Article
  9. Biotechnology reports (Amsterdam, Netherlands) · 2020
    Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 3 countries.

Sujan KhadkaDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan University, Chitwan, Bharatpur, 44200, Nepal.ORCID http://orcid.org/0000-0003-1451-7804
Sanjib AdhikariDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan University, Chitwan, Bharatpur, 44200, Nepal. sanadh26@gmail.com.ORCID http://orcid.org/0000-0002-5874-2547
Alina ThapaState Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, 100101, China.ORCID http://orcid.org/0000-0003-2866-5103
Raju PandayDepartment of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
Manjila AdhikariDepartment of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
Sanjeep SapkotaDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan University, Chitwan, Bharatpur, 44200, Nepal.
Ramesh Sharma RegmiDepartment of Microbiology, Birendra Multiple Campus, Tribhuvan University, Chitwan, Bharatpur, 44200, Nepal.
Namita Paudel AdhikariState Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, 100101, China.
Ram ProshadKey Laboratory of Mountain Surface Process and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, 610041, China.
Niranjan KoiralaDepartment of Natural Products Research, Dr. Koirala Research Institute for Biotechnology and Biodiversity, Kathmandu, 44613, Nepal.ORCID http://orcid.org/0000-0002-7777-1191
Tribhuvan University · NPChinese Academy of Sciences · CNHuazhong University of Science and Technology · CNUniversity of Macau · MO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The current study was designed to isolate, identify and characterize a Bacillus sp. capable of producing protease and exhibiting antifungal activity. A highly potent bacterium capable of producing protease abundantly was isolated from the soil collected from the waste pit near Microbiology Laboratory of Birendra Multiple Campus, Bharatpur and later on identified as Lysinibacillus fusiformis strain SK on the basis of morphological, physiological, biochemical and 16S rDNA gene sequencing techniques. The strain SK showed 98.36% similarity with L. fusiformis strain NBRC 15717. Using R-programming statistical analysis tool, the optimum incubation time for the highest average protease production (APP) (47.2 U/mL) was found to be 22 h at 50 °C and both incubation time and temperature showed a significant impact on the production of protease (P < 0.01). The maximum average relative protease activity (ARPA) was observed at pH 7.8 and 48 °C, whereas the least ARPA was observed in the presence of 80 g/L NaCl and 10 g/L HgCl

Indexed as

AntibiosisSoil MicrobiologyAspergillusAspergillus flavusBacillaceaeBacterial Typing TechniquesDNA, BacterialNepalPeptide HydrolasesPhylogenyRNA, Ribosomal, 16SSequence Analysis, DNADNA, BacterialPeptide HydrolasesRNA, Ribosomal, 16S

Identifiers

PMID32248284
OpenAlexW3014382068

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.