Evidence map›Paper›PMID 32247863›Full record

ArticleThe Journal of molecular diagnostics : JMD2020

Analytical Evaluation of the Abbott RealTime CT/NG Assay for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Rectal and Pharyngeal Swabs.

Paul C Adamson, Mark W Pandori, Sarah B Doernberg, Lauren Komarow, Zoe Sund, Thuy Tien T Tran, David Jensen, Ephraim L Tsalik, Carolyn D Deal, Henry F Chambers and 5 more

Open access · hybridAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of molecular diagnostics : JMD, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.3field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Visual and rapid identification ofFrontiers in cellular and infection microbiology · 2023
    Article
  3. Review
  4. High Prevalence of RectalOpen forum infectious diseases · 2022
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 7 institutions in 1 country.

Paul C AdamsonDivision of Infectious Diseases, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California. Electronic address: padamson@mednet.ucla.edu.
Mark W PandoriAlameda County Department of Public Health, Oakland, California.
Sarah B DoernbergDivision of Infectious Diseases, School of Medicine, University of California, San Francisco, San Francisco, California.
Lauren KomarowThe Biostatistics Center, George Washington University, Rockville Maryland.
Zoe SundOffice of Regulatory Affairs and Quality, School of Medicine, Duke University, Durham, North Carolina.
Thuy Tien T TranThe Biostatistics Center, George Washington University, Rockville Maryland.
David JensenOffice of Regulatory Affairs and Quality, School of Medicine, Duke University, Durham, North Carolina.
Ephraim L TsalikDivision of Infectious Diseases, Department of Medicine, Duke University, Durham, North Carolina; Emergency Medicine Service, Durham Veterans Affairs Medical Center, Durham, North Carolina.
Carolyn D DealEnteric and Sexually Transmitted Infections Branch, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland.
Henry F ChambersDivision of Infectious Diseases, School of Medicine, University of California, San Francisco, San Francisco, California.
Vance G FowlerOffice of Regulatory Affairs and Quality, School of Medicine, Duke University, Durham, North Carolina.
Scott R EvansThe Biostatistics Center, George Washington University, Rockville Maryland.
Robin PatelDivision of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota.
Jeffrey D KlausnerDivision of Infectious Diseases, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California; Department of Epidemiology, Fielding School of Public Health, University of California, Los Angeles, Los Angeles, California. Electronic address: jdklausner@mednet.ucla.edu.
Antibacterial Resistance Leadership Group
Duke University · USGeorge Washington University · USUniversity of California, Los Angeles · USUniversity of California, San Francisco · USAlameda County Public Health Department · USMayo Clinic · USNational Institute of Allergy and Infectious Diseases · US

Funding

Antibacterial Resistance Leadership Group (ARLG)UM1AI104681 · NIAID · DUKE UNIVERSITY · PI Vance G. Fowler, Henry F HENRY CHAMBERS · 2013 to 2026
$180.8M
Postdoctoral Training in Global AIDS Prevention ResearchT32MH080634 · NIMH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JESSE LAWTON CLARK, Pamina Mae Gorbach · 2007 to 2026
$6.4M
Mentoring Patient Oriented Research in Antibiotic-Resistant BacteriaK24AI093969 · NIAID · DUKE UNIVERSITY · PI FOWLER, VANCE G. · 2011 to 2020
$1.6M
NIAID NIH HHS K24 AI093969NIAID NIH HHS UM1 AI104681NIMH NIH HHS T32 MH080634
6 · The paper itself

Abstract

Chlamydia trachomatis and Neisseria gonorrhoeae infections in the rectum and pharynx are important extragenital reservoirs of infection. Few assays approved by the US Food and Drug Administration are commercially available to diagnose pharyngeal or rectal infections. The current study reports on the analytical performance of the Abbott RealTime CT/NG assay, including the limit of detection, inclusivity, and analytical specificity for C. trachomatis and N. gonorrhoeae in rectal and pharyngeal specimens. The limit of detection was performed using known concentrations of organisms, elementary bodies per milliliter (EB/mL) for C. trachomatis and colony-forming units per milliliter (CFU/mL) for N. gonorrhoeae, in clinical rectal and pharyngeal swab matrices. Inclusivity was performed against 12 serovars of C. trachomatis and seven strains of N. gonorrhoeae. The analytical specificity was performed using 28 different bacteria and viruses. The limit of detection for C. trachomatis was 2.56 EB/mL in pharyngeal specimens and 12.8 EB/mL in rectal specimens. The limit of detection for N. gonorrhoeae was 0.0256 CFU/mL for both pharyngeal and rectal specimens. The inclusivity and analytical specificity were 100% for both rectal and pharyngeal specimens. These analytical performance data demonstrate that the Abbott CT/NG RealTime assay is an accurate, sensitive, and specific assay in rectal and pharyngeal specimens, supporting the potential of the assay for detection of rectal and pharyngeal C. trachomatis and N. gonorrhoeae infections.

Indexed as

Chlamydia InfectionsChlamydia trachomatisData AccuracyFemaleGonorrheaHumansLimit of DetectionMaleNeisseria gonorrhoeaePharynxReal-Time Polymerase Chain ReactionRectumSensitivity and Specificity

Identifiers

PMID32247863
PMCPMC7295135
OpenAlexW3014945409

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.