Evidence map›Paper›PMID 32246987›Full record

ArticleMetabolism: clinical and experimental2020

DDB1 E3 ligase controls dietary fructose-induced ChREBPα stabilization and liver steatosis via CRY1.

Xin Tong, Deqiang Zhang, Omar Shabandri, Joon Oh, Ethan Jin, Kenneth Stamper, Meichan Yang, Zifeng Zhao, Lei Yin

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Metabolism: clinical and experimental, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.4field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. The E3 Ligase RNF8 Promotes Ubiquitination and Degradation of ChREBPα During Liver Stress Response.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
    Article
  3. Targeting Cryptochromes in Chronic Diseases.Cell biology international · 2025
    Review
  4. Article
  5. Article
  6. Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Xin TongDepartment of Molecular & Integrative Physiology, University of Michigan Medical School, 1137 Catherine Street, Med Sci II 7712, Ann Arbor, MI 48109, USA.
Deqiang ZhangDepartment of Molecular & Integrative Physiology, University of Michigan Medical School, 1137 Catherine Street, Med Sci II 7712, Ann Arbor, MI 48109, USA.
Omar ShabandriDepartment of Molecular & Integrative Physiology, University of Michigan Medical School, 1137 Catherine Street, Med Sci II 7712, Ann Arbor, MI 48109, USA.
Joon OhDepartment of Molecular & Integrative Physiology, University of Michigan Medical School, 1137 Catherine Street, Med Sci II 7712, Ann Arbor, MI 48109, USA.
Ethan JinDepartment of Molecular & Integrative Physiology, University of Michigan Medical School, 1137 Catherine Street, Med Sci II 7712, Ann Arbor, MI 48109, USA.
Kenneth StamperDepartment of Molecular & Integrative Physiology, University of Michigan Medical School, 1137 Catherine Street, Med Sci II 7712, Ann Arbor, MI 48109, USA.
Meichan YangDepartment of Molecular & Integrative Physiology, University of Michigan Medical School, 1137 Catherine Street, Med Sci II 7712, Ann Arbor, MI 48109, USA; Department of Infectious Diseases, The Second Xianya Hospital, Central South University, Changsha City 410083, Hunan Province, PR China.
Zifeng ZhaoDepartment of Molecular & Integrative Physiology, University of Michigan Medical School, 1137 Catherine Street, Med Sci II 7712, Ann Arbor, MI 48109, USA; Department of Pharmacology of Chinese Materia, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing City 211198, PR China.
Lei YinDepartment of Molecular & Integrative Physiology, University of Michigan Medical School, 1137 Catherine Street, Med Sci II 7712, Ann Arbor, MI 48109, USA. Electronic address: leiyin@umich.edu.
University of Michigan–Ann Arbor · USCentral South University · CN

Funding

PUBLIC HEALTH DEMONSTRATIONP60DK020572 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MYERS, MARTIN G · 1985 to 2012
$26.5M
Regional Pilot And Feasibility Study Grants ProgramP30DK020572 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Scott Soleimanpour · 2013 to 2026
$24.3M
University of Michigan Center for Gastrointestinal ResearchP30DK034933 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI OWYANG, CHUNG · 1986 to 2021
$22.5M
Pilot and Feasibility (P and F) ProgramP30DK089503 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Joyce Lee · 2010 to 2026
$20.3M
Significance and mechanisms of hepatic ChREBPα induction via post-translational modifications in diet-induced NASHR01DK099593 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI YIN, LEI · 2014 to 2025
$4.5M
Role and Mechanisms of E4BP4 over-activation in diet-induced fatty liver diseaseR01DK121170 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI TONG, XIN · 2019 to 2022
$1.6M
Biology of nuclear receptor Rev-erb alpha in circadian rhythm and metabolismR00DK077449 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI YIN, LEI · 2009 to 2011
$724k
Biology of nuclear receptor rev-erb alpha in circadian rhythm and metabolismK99DK077449 · NIDDK · UNIVERSITY OF PENNSYLVANIA · PI YIN, LEI · 2007 to 2009
$146k
NIDDK NIH HHS K99 DK077449NIDDK NIH HHS P30 DK020572NIDDK NIH HHS P30 DK034933NIDDK NIH HHS P30 DK089503NIDDK NIH HHS P60 DK020572NIDDK NIH HHS R00 DK077449NIDDK NIH HHS R01 DK099593NIDDK NIH HHS R01 DK121170
6 · The paper itself

Abstract

Fructose over-consumption contributes to the development of liver steatosis in part by stimulating ChREBPα-driven de novo lipogenesis. However, the mechanisms by which fructose activates ChREBP pathway remain largely undefined. Here we performed affinity purification of ChREBPα followed by mass spectrometry and identified DDB1 as a novel interaction protein of ChREBPα in the presence of fructose. Depletion and overexpression of Ddb1 showed opposite effects on the ChREBPα stability in hepatocytes. We next tested the impact of hepatic Ddb1 deficiency on the fructose-induced ChREBP pathway. After 3-week high-fructose diet feeding, both Ddb1 liver-specific knockout and AAV-TBG-Cre-injected Ddb1

Indexed as

AnimalsBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsCryptochromesDietary CarbohydratesDNA-Binding ProteinsFatty LiverFructoseHepatocytesImmediate-Early ProteinsLipogenesisMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutMutationPrimary Cell CultureBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsCry1 protein, mouseCryptochromesDdb1 protein, mouseDietary CarbohydratesDNA-Binding ProteinsFructoseImmediate-Early ProteinsLaptm5 protein, mouseMembrane ProteinsMlxipl protein, mouseChREBPαCRY1DDB1De novo lipogenesisFructoseLiver steatosis

Identifiers

PMID32246987
PMCPMC7282961
OpenAlexW3014606248

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.