ArticleNucleic acids research2020
Development of a genetic toolset for the highly engineerable and metabolically versatile Acinetobacter baylyi ADP1.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Resilience of recombinant antibiotic resistance gene-containing plasmids against common cell culture disposal methods.Cell reports methods · 2026Article
- Article
- Genome editing of phylogenetically distinct bacteria using cross-species retron-mediated recombineering.Nature biotechnology · 2026Article
- A WYL transcriptional regulator activates the DNA damage response pathway in Acinetobacter species.Nucleic acids research · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Characterization of carbon metabolism in a highly adhesive bacteriumFrontiers in microbiology · 2026Article
- Expression of a phage RecET recombinase system improves detection of shorter DNA fragments by anmicroPublication biology · 2026Article
- Engineering microbial consortia for mixed plastic upcycling.Nature communications · 2025Article
- Rapid accumulation of fluorophores and fast kill identify drugs with bactericidal effects against Gram-negative bacteria.Microbiology (Reading, England) · 2025Article
- Rewiring Aromatic Compound Consumption: Chromosomal Amplification and Evolution of a Foreign Pathway inACS synthetic biology · 2025Article
- Cis,cis-muconic acid production from lignin related molecules byAcinetobacter baylyi ADP1.Microbial cell factories · 2025Article
- Metabolic engineering ofMetabolic engineering communications · 2024Article
- Regulation of tricarboxylate transport and metabolism inApplied and environmental microbiology · 2024Article
- Microbial Cell Factories in the Bioeconomy Era: From Discovery to Creation.Biodesign research · 2024Review
- Genetic characterization of the cyclohexane carboxylate degradation pathway in the denitrifying bacterium Aromatoleum sp. CIB.Environmental microbiology · 2022Article
- Engineering cell morphology by CRISPR interference in Acinetobacter baylyi ADP1.Microbial biotechnology · 2022Article
- A Framework for the Systematic Selection of Biosensor Chassis for Environmental Synthetic Biology.ACS synthetic biology · 2022Review
- Regulation of l- and d-Aspartate Transport and Metabolism in Acinetobacter baylyi ADP1.Applied and environmental microbiology · 2022Article
- Characterization of Highly Ferulate-Tolerant Acinetobacter baylyi ADP1 Isolates by a Rapid Reverse Engineering Method.Applied and environmental microbiology · 2022Article
- EngineeringMetabolic engineering communications · 2021Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
One primary objective of synthetic biology is to improve the sustainability of chemical manufacturing. Naturally occurring biological systems can utilize a variety of carbon sources, including waste streams that pose challenges to traditional chemical processing, such as lignin biomass, providing opportunity for remediation and valorization of these materials. Success, however, depends on identifying micro-organisms that are both metabolically versatile and engineerable. Identifying organisms with this combination of traits has been a historic hindrance. Here, we leverage the facile genetics of the metabolically versatile bacterium Acinetobacter baylyi ADP1 to create easy and rapid molecular cloning workflows, including a Cas9-based single-step marker-less and scar-less genomic integration method. In addition, we create a promoter library, ribosomal binding site (RBS) variants and test an unprecedented number of rationally integrated bacterial chromosomal protein expression sites and variants. At last, we demonstrate the utility of these tools by examining ADP1's catabolic repression regulation, creating a strain with improved potential for lignin bioprocessing. Taken together, this work highlights ADP1 as an ideal host for a variety of sustainability and synthetic biology applications.
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