ArticleAngewandte Chemie (International ed. in English)2020
One-Step Nucleic Acid Purification and Noise-Resistant Polymerase Chain Reaction by Electrokinetic Concentration for Ultralow-Abundance Nucleic Acid Detection.
Article in Angewandte Chemie (International ed. in English), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Article
- Open and closed microfluidics for biosensing.Materials today. Bio · 2024Review
- Manipulation of magnetic beads for actively capturingBiomicrofluidics · 2024Article
- Advances in Simple, Rapid, and Contamination-Free Instantaneous Nucleic Acid Devices for Pathogen Detection.Biosensors · 2023Review
- CRISPR/Cas12a-Enabled Multiplex Biosensing Strategy Via an Affordable and Visual Nylon Membrane Readout.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics.Trends in analytical chemistry : TRAC · 2023Review
- ABiosensors · 2022Article
- Analytical Technologies for Liquid Biopsy of Subcellular Materials.Annual review of analytical chemistry (Palo Alto, Calif.) · 2021Article
- A HiPAD Integrated with rGO/MWCNTs Nano-Circuit Heater for Visual Point-of-Care Testing of SARS-CoV-2.Advanced functional materials · 2021Article
- Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk.Micromachines · 2021Review
- Point-of-Care PCR Assays for COVID-19 Detection.Biosensors · 2021Review
- CovidArray: A Microarray-Based Assay with High Sensitivity for the Detection of Sars-Cov-2 in Nasopharyngeal Swabs.Sensors (Basel, Switzerland) · 2021Article
- Antigen tests for COVID-19.Biophysics and physicobiology · 2021Article
- Detection of COVID-19: A review of the current literature and future perspectives.Biosensors & bioelectronics · 2020Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Nucleic acid amplification tests (NAATs)integrated on a chip hold great promise for point-of-care diagnostics. Currently, nucleic acid (NA) purification remains time-consuming and labor-intensive, and it takes extensive efforts to optimize the amplification chemistry. Using selective electrokinetic concentration, we report one-step, liquid-phase NA purification that is simpler and faster than conventional solid-phase extraction. By further re-concentrating NAs and performing polymerase chain reaction (PCR) in a microfluidic chamber, our platform suppresses non-specific amplification caused by non-optimal PCR designs. We achieved the detection of 5 copies of M. tuberculosis genomic DNA (equaling 0.3 cell) in real biofluids using both optimized and non-optimal PCR designs, which is 10- and 1000-fold fewer than those of the standard bench-top method, respectively. By simplifying the workflow and shortening the development cycle of NAATs, our platform may find use in point-of-care diagnosis.
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Registered trials
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