ArticleMetabolomics : Official journal of the Metabolomic Society2020
An LC-QToF MS based method for untargeted metabolomics of human fecal samples.
Article in Metabolomics : Official journal of the Metabolomic Society, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- A Validated LC-MS/MS Method for Quantifying Phenolic Acids, Lignans, and Enterolignans from Human Fecal Samples.ACS nutrition science · 2026Article
- Mass spectrometry-based metabolomics for studying gut microbiota-derived metabolites in biological samples.Journal of food and drug analysis · 2026Review
- Article
- Acute myocardial infarction induces sex-specific, time-dependent remodeling of the gut microbiome and intestinal immune compartment in retired breeder C57BL/6N mice.Frontiers in microbiomes · 2026Article
- Development of an untargeted metabolomics analytical protocol for fecal samples by liquid chromatography-mass spectrometry.Journal of food and drug analysis · 2025Article
- From Complexity to Clarity: Expanding Metabolome Coverage With Innovative Analytical Strategies.Journal of separation science · 2025Review
- The gut metabolome in a cohort of pregnant and lactating women from Antioquia-Colombia.Frontiers in molecular biosciences · 2024Article
- An untargeted comparative metabolomics analysis of infants with and without late-onset breast milk jaundice.PloS one · 2024Article
- The metagenomic and metabolomic profile of the gut microbes in Chinese full-term and late preterm infants treated with Clostridium butyricum.Scientific reports · 2023Article
- Gut Microbiota Enterotypes Mediate the Effects of Dietary Patterns on Colorectal Neoplasm Risk in a Chinese Population.Nutrients · 2023Article
- An Optimised Monophasic Faecal Extraction Method for LC-MS Analysis and Its Application in Gastrointestinal Disease.Metabolites · 2022Article
- Positive Interventional Effect of Engineered Butyrate-Producing Bacteria on Metabolic Disorders and Intestinal Flora Disruption in Obese Mice.Microbiology spectrum · 2022Article
- Evaluation of fresh, frozen, and lyophilized fecal samples by SPME and derivatization methods using GC×GC-TOFMS.Metabolomics : Official journal of the Metabolomic Society · 2022Article
- AUF-1 knockdown in mice undermines gut microbial butyrate-driven hypocholesterolemia through AUF-1-Dicer-1-mir-122 hierarchy.Frontiers in cellular and infection microbiology · 2022Article
- Advances in experimental and computational methodologies for the study of microbial-surface interactions at different omics levels.Frontiers in microbiology · 2022Review
- Rapid ex vivo molecular fingerprinting of biofluids using laser-assisted rapid evaporative ionization mass spectrometry.Nature protocols · 2021Article
- Article
- Safety of high-doseFood science & nutrition · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionConsensus in sample preparation for untargeted human fecal metabolomics is lacking.
objectivesTo obtain sample preparation with broad metabolite coverage for high-throughput LC-MS.
methodsExtraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by metabolite feature quality.
resultsMethanol at 5 mL per g wet feces provided a wide metabolite coverage with optimal balance between signal intensity and saturation for both fresh frozen and lyophilized samples. Lyophilization did not affect SCFA and is recommended because of convenience in normalizing to dry matter.
conclusionThe suggested sample preparation is simple, efficient and suitable for large-scale human fecal metabolomics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.