ArticleNature communications2020
Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed, 154 citations in OpenAlex.
- Adaptive Laboratory Evolution inMicroorganisms · 2026Review
- Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression.Current issues in molecular biology · 2026Review
- Cross-Strain Transferability of CRISPRi Systems and Design Rules from Laboratory to ClinicalACS synthetic biology · 2026Article
- CRISPRi-based functional genomic screening identifies genes essential for CHScience advances · 2026Article
- From pond to platform: howJournal of bacteriology · 2026Review
- Deciphering the metabolic details of L-lysine toxicity in cyanobacteria.Plant physiology · 2026Article
- Genetically encoded biosensors enabled high-throughput screening of microbial cell factories.Engineering microbiology · 2026Review
- Cross-strain transferability of CRISPRi systems and design rules from laboratory to clinicalbioRxiv : the preprint server for biology · 2026Article
- Comprehensive genetic interaction analysis of the Bacillus subtilis envelope using double-CRISPRi.Cell systems · 2025Article
- The quintessence of algal biomass in bioplastic production: insightful advancement and sustainable use.Bioresources and bioprocessing · 2025Review
- Establishing a Malonyl-CoA Biosensor for the Two Model CyanobacteriaACS synthetic biology · 2025Article
- Development of a CRISPR activation system for targeted gene upregulation in Synechocystis sp. PCC 6803.Communications biology · 2025Article
- Article
- Genome-scale CRISPRi screen identifies pcnB repression conferring improved physiology for overproduction of free fatty acids in Escherichia coli.Nature communications · 2025Article
- High-density CRISPRi screens reveal diverse routes to improved acclimation in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Outlook on Synthetic Biology-Driven Hydrogen Production: Lessons from Algal Photosynthesis Applied to Cyanobacteria.Energy & fuels : an American Chemical Society journal · 2025Review
- An automated high-throughput lighting system for screening photosynthetic microorganisms in plate-based formats.Communications biology · 2025Article
- How Small Proteins Adjust the Metabolism of Cyanobacteria Under Stress: The Role of Small Proteins in Cyanobacterial Stress Responses.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Photosynthesis in Synechocystis sp. PCC 6803 is not optimally regulated under very high COApplied microbiology and biotechnology · 2025Article
- Engineering a Photoautotrophic Microbial Coculture toward Enhanced Biohydrogen Production.Environmental science & technology · 2025Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanobacteria are model organisms for photosynthesis and are attractive for biotechnology applications. To aid investigation of genotype-phenotype relationships in cyanobacteria, we develop an inducible CRISPRi gene repression library in Synechocystis sp. PCC 6803, where we aim to target all genes for repression. We track the growth of all library members in multiple conditions and estimate gene fitness. The library reveals several clones with increased growth rates, and these have a common upregulation of genes related to cyclic electron flow. We challenge the library with 0.1 M L-lactate and find that repression of peroxiredoxin bcp2 increases growth rate by 49%. Transforming the library into an L-lactate-secreting Synechocystis strain and sorting top lactate producers enriches clones with sgRNAs targeting nutrient assimilation, central carbon metabolism, and cyclic electron flow. In many examples, productivity can be enhanced by repression of essential genes, which are difficult to access by transposon insertion.
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