ArticleProceedings of the National Academy of Sciences of the United States of America2020
Modular repeat protein sculpting using rigid helical junctions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Designing novel solenoid proteins with in silico evolution.Communications chemistry · 2025Article
- Designed miniproteins potently inhibit and protect against MERS-CoV.Cell reports · 2025Article
- From bench to bedside: cutting-edge applications of base editing and prime editing in precision medicine.Journal of translational medicine · 2024Review
- Design of pseudosymmetric protein hetero-oligomers.Nature communications · 2024Article
- Designed miniproteins potently inhibit and protect against MERS-CoV.bioRxiv : the preprint server for biology · 2024Article
- De novo design of buttressed loops for sculpting protein functions.Nature chemical biology · 2024Article
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- De novo design of proteins housing excitonically coupled chlorophyll special pairs.Nature chemical biology · 2024Article
- De novo design of alpha-beta repeat proteins.bioRxiv : the preprint server for biology · 2024Article
- Article
- Design of stimulus-responsive two-state hinge proteins.Science (New York, N.Y.) · 2023Article
- Article
- Article
- Modulation of FGF pathway signaling and vascular differentiation using designed oligomeric assemblies.bioRxiv : the preprint server for biology · 2023Article
- De novo design of small beta barrel proteins.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- De novo design of immunoglobulin-like domains.Nature communications · 2022Article
- Catalysis and Electron Transfer inChemical reviews · 2022Review
- Article
- Reconfigurable asymmetric protein assemblies through implicit negative design.Science (New York, N.Y.) · 2022Article
- Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The ability to precisely design large proteins with diverse shapes would enable applications ranging from the design of protein binders that wrap around their target to the positioning of multiple functional sites in specified orientations. We describe a protein backbone design method for generating a wide range of rigid fusions between helix-containing proteins and use it to design 75,000 structurally unique junctions between monomeric and homo-oligomeric de novo designed and ankyrin repeat proteins (RPs). Of the junction designs that were experimentally characterized, 82% have circular dichroism and solution small-angle X-ray scattering profiles consistent with the design models and are stable at 95 °C. Crystal structures of four designed junctions were in close agreement with the design models with rmsds ranging from 0.9 to 1.6 Å. Electron microscopic images of extended tetrameric structures and ∼10-nm-diameter "L" and "V" shapes generated using the junctions are close to the design models, demonstrating the control the rigid junctions provide for protein shape sculpting over multiple nanometer length scales.
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