ArticleVirology journal2020
Identification of a novel B-cell epitope in the spike protein of porcine epidemic diarrhea virus.
Article in Virology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 30 citations in OpenAlex.
- A colloidal gold immunochromatographic test strip based on McAbs anti-S protein to detect porcine epidemic diarrhea virus.Microbiology spectrum · 2026Article
- A monoclonal antibody-based electrochemical immunosensor for porcine epidemic diarrhea virus.Applied microbiology and biotechnology · 2026Article
- A needle-free intradermal PEDV S protein vaccine elicits protective lactogenic immunity in piglets.Frontiers in cellular and infection microbiology · 2026Article
- A novel genotyping system based on site polymorphism on spike gene reveals the evolutionary pathway of porcine epidemic diarrhea virus.iMetaOmics · 2025Article
- Current Evolutionary Dynamics of Porcine Epidemic Diarrhea Virus (PEDV) in the U.S. a Decade After Introduction.Viruses · 2025Article
- Isolation of porcine epidemic diarrhea virus strain CHCQ-2023 from Chongqing Province and analysis of S gene recombination.BMC veterinary research · 2024Article
- A Nanobody of PEDV S1 Protein: Screening and Expression inBiomolecules · 2024Article
- Identification of a New B-Cell Epitope on the Capsid Protein of Avian Leukosis Virus and Its Application.Current issues in molecular biology · 2024Article
- Article
- Research progress of porcine epidemic diarrhea virus S protein.Frontiers in microbiology · 2024Review
- Identification of a Novel Linear B-Cell Epitope of HbpA Protein fromInternational journal of molecular sciences · 2023Article
- Antigen epitopes of animal coronaviruses: a mini-review.Animal diseases · 2023Review
- Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus strains circulating in China from 2020 to 2021.BMC veterinary research · 2022Article
- Current State of Molecular and Serological Methods for Detection of Porcine Epidemic Diarrhea Virus.Pathogens (Basel, Switzerland) · 2022Review
- Antigenicity Alternations of Variant PEDV S Protein Disclosed by Linear B Cell Epitope Mapping.Viruses · 2022Article
- Article
- EGR1 Suppresses Porcine Epidemic Diarrhea Virus Replication by Regulating IRAV To Degrade Viral Nucleocapsid Protein.Journal of virology · 2021Article
- Genetic characterization and phylogenetic analysis of porcine epidemic diarrhea virus in Guangdong, China, between 2018 and 2019.PloS one · 2021Article
- Immunodominant and Neutralizing Linear B-Cell Epitopes Spanning the Spike and Membrane Proteins of Porcine Epidemic Diarrhea Virus.Frontiers in immunology · 2021Article
- Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis.International journal of biological sciences · 2021Article
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundPorcine epidemic diarrhea virus (PEDV) infection causes an acute enteric tract infectious disease characterized by vomiting, anorexia, dehydration, weight loss and high mortality in neonatal piglets. During PEDV infection, the spike protein (S) is a major virion structural protein interacting with receptors and inducing neutralizing antibodies. However, the neutralizing B-cell epitopes within PEDV S protein have not been well studied.
methodsTo accurately identify the important immunodominant region of S1, the purified truncated S1 proteins (SA, SB, SC, SD and SE) were used to immunize BALB/c mice to prepare polyclonal antibodies. The antisera titers were determined by indirect ELISA, western blot and IFA after four immunizations to find the important immunodominant region of S1, and then purified the immunodominant region of S1 protein and immunized mice to generate the special antibodies, and then used recombinant peptides to determine the B-cell epitopes of monoclonal antibodies.
resultsFive antisera of recombinant proteins of the spike protein region of PEDV were generated and we found that only the polyclonal antibody against part of the S1 region (signed as SE protein, residues 666-789) could recognize the native PEDV. Purified SE protein was used to immunize BALB/c mice and generate mAb 2E10. Pepscan of the SE protein demonstrated that SE16 (
conclusionsA mAb 2E10 that is specifically bound to PEDV was generated and identified a specific linear B-cell epitope (SE16,
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