Evidence map›Paper›PMID 32245408›Full record

ArticleBMC molecular and cell biology2020

PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth.

Bashar M Thejer, Partho P Adhikary, Amandeep Kaur, Sarah L Teakel, Ashleigh Van Oosterum, Ishith Seth, Marina Pajic, Katherine M Hannan, Megan Pavy, Perlita Poh and 21 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC molecular and cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
6.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 44 citations in OpenAlex.

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  10. Cancer cell metabolic plasticity in migration and metastasis.Clinical & experimental metastasis · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

31 authors at 16 institutions in 5 countries.

Bashar M ThejerSchool of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, 2650, Australia.
Partho P AdhikarySchool of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, 2650, Australia.
Amandeep KaurSchool of Chemistry, University of Sydney, Sydney, NSW, 2006, Australia.
Sarah L TeakelSchool of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, 2650, Australia.
Ashleigh Van OosterumLife Sciences and Health, Faculty of Science, Charles Sturt University, Wagga Wagga, NSW, 2650, Australia.
Ishith SethSchool of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, 2650, Australia.
Marina PajicThe Kinghorn Cancer Centre, Cancer Division, Garvan Institute of Medical Research, 384 Victoria St, Darlinghurst, Sydney, NSW, 2010, Australia.
Katherine M HannanDepartment of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria, 3010, Australia.
Megan PavyACRF Department of Cancer Biology and Therapeutics, The John Curtin School of Medical Research, The Australian National University, ACT, Canberra, 2601, Australia.
Perlita PohACRF Department of Cancer Biology and Therapeutics, The John Curtin School of Medical Research, The Australian National University, ACT, Canberra, 2601, Australia.
Jalal A JazayeriSchool of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, 2650, Australia.
Thiri ZawAustralian Proteome Analysis Facility, Macquarie University, Sydney, NSW, 2109, Australia.
Dana PascoviciAustralian Proteome Analysis Facility, Macquarie University, Sydney, NSW, 2109, Australia.
Marina LudescherDepartment of Gynecology and Obstetrics, University Women's Hospital of Dusseldorf, 40225, Dusseldorf, Germany.
Michael PawlakNMI TT Pharmaservices, Protein Profiling, 72770, Reutlingen, Germany.
Juan C CassanoParticles-Biology Interactions Laboratory, Department of Materials Meet Life, Swiss Federal Laboratories for Materials Science & Technology (Empa), Lerchenfeldstrasse 5, CH-9014, St Gallen, Switzerland.
Lynne TurnbullThe ithree institute, University of Technology Sydney, Ultimo, NSW, 2007, Australia.
Mitra JazayeriDepartment of Mathematics and Statistics, La Trobe University, Melbourne, VIC, 3086, Australia.
Alexander C JamesIngham Institute for Applied Medical Research, Liverpool, NSW, 2170, Australia.
Craig P CooreyIngham Institute for Applied Medical Research, Liverpool, NSW, 2170, Australia.
Tara L RobertsIngham Institute for Applied Medical Research, Liverpool, NSW, 2170, Australia.
Simon J KinderAXT Pty Ltd, Warriewood (Sydney), NSW, 2102, Australia.
Ross D HannanDepartment of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria, 3010, Australia.
Ellis PatrickSchool of Mathematics and Statistics, University of Sydney, Sydney, NSW, 2006, Australia.
Mark P MolloyAustralian Proteome Analysis Facility, Macquarie University, Sydney, NSW, 2109, Australia.
Elizabeth J NewSchool of Chemistry, University of Sydney, Sydney, NSW, 2006, Australia.
Tanja N FehmDepartment of Gynecology and Obstetrics, University Women's Hospital of Dusseldorf, 40225, Dusseldorf, Germany.
Hans NeubauerDepartment of Gynecology and Obstetrics, University Women's Hospital of Dusseldorf, 40225, Dusseldorf, Germany.
Ewa M GoldysARC Centre of Excellence for Nanoscale BioPhotonics, Macquarie University, Sydney, NSW, 2109, Australia.
Leslie A WestonGraham Centre for Agricultural Innovation, Charles Sturt University, Boorooma Street, Wagga Wagga, NSW, 2678, Australia.
Michael A CahillSchool of Biomedical Sciences, Charles Sturt University, Wagga Wagga, NSW, 2650, Australia. mcahill@csu.edu.au.
Charles Sturt University · AUAustralian National University · AUDüsseldorf University Hospital · DEMacquarie University · AUThe University of Sydney · AUSouth Western Sydney Local Health District · AUAustralian Cancer Research Foundation · AULa Trobe University · AUNatural and Medical Sciences Institute · DESt Vincent's Clinic · AUSwiss Federal Laboratories for Materials Science and Technology · CHThe University of Queensland · AUUniversity of British Columbia · CAUniversity of Technology Sydney · AUUniversity of Wasit · IQUNSW Sydney · AU

Funding

Australian Research Council CE140100003Australian Research Council (AU) DE120102687Cancer Institute NSW Career Development FellowshipCancer Institute NSW (AU) Future Research Leader FellowshipCharles Sturt University N/AIraqi Cultural Attaché in Canberra (AUS) N/ANational Health and Medical Research Council RD Wright Biomedical Career Development FellowshipRamaciotti Foundations ES2012/0051University of Sydney World Scholars Scholarship
6 · The paper itself

Abstract

backgroundProgesterone Receptor Membrane Component 1 (PGRMC1) is expressed in many cancer cells, where it is associated with detrimental patient outcomes. It contains phosphorylated tyrosines which evolutionarily preceded deuterostome gastrulation and tissue differentiation mechanisms.

resultsWe demonstrate that manipulating PGRMC1 phosphorylation status in MIA PaCa-2 (MP) cells imposes broad pleiotropic effects. Relative to parental cells over-expressing hemagglutinin-tagged wild-type (WT) PGRMC1-HA, cells expressing a PGRMC1-HA-S57A/S181A double mutant (DM) exhibited reduced levels of proteins involved in energy metabolism and mitochondrial function, and altered glucose metabolism suggesting modulation of the Warburg effect. This was associated with increased PI3K/AKT activity, altered cell shape, actin cytoskeleton, motility, and mitochondrial properties. An S57A/Y180F/S181A triple mutant (TM) indicated the involvement of Y180 in PI3K/AKT activation. Mutation of Y180F strongly attenuated subcutaneous xenograft tumor growth in NOD-SCID gamma mice. Elsewhere we demonstrate altered metabolism, mutation incidence, and epigenetic status in these cells.

conclusionsAltogether, these results indicate that mutational manipulation of PGRMC1 phosphorylation status exerts broad pleiotropic effects relevant to cancer and other cell biology.

Indexed as

PhosphorylationReceptors, ProgesteroneAnimalsCell Line, TumorCell MovementCell ProliferationCell ShapeEnergy MetabolismGlycolysisHumansMembrane ProteinsMiceMice, Inbred NODMice, SCIDMitochondriaNeoplasmsMembrane ProteinsPGRMC1 protein, humanPGRMC1 protein, mousePhosphatidylinositol 3-KinasesReceptors, ProgesteroneCytochrome P450InvasionMesenchymal amoeboid transitionMetabolismMigrationMitochondriaProteomicsTumor biology

Identifiers

PMID32245408
PMCPMC7119165
OpenAlexW3014781092

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.