ArticleMicroorganisms2020
Identification and in vitro Characterization of a Novel Phage Endolysin that Targets Gram-Negative Bacteria.
Article in Microorganisms, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Genomic characterization of Pseudomonas aeruginosa infecting Abidjanvirus Lucjan.Molecular genetics and genomics : MGG · 2026Article
- Avian PathogenicVeterinary sciences · 2025Review
- Tuning and functionalization of logic gates for time resolved programming of bacterial populations.Nucleic acids research · 2025Article
- Endolysins and membrane-active peptides: innovative engineering strategies against gram-negative bacteria.Frontiers in microbiology · 2025Review
- Characterization and genomic analysis of twoFrontiers in microbiology · 2025Article
- SXP01: a novel bacteriophage for combatingFrontiers in microbiology · 2025Article
- Elucidation of molecular function of phage protein responsible for optimization of host cell lysis.BMC microbiology · 2024Article
- Reclassification of the first Bacillus tropicus phage calls for reclassification of other Bacillus temperate phages previously designated as plasmids.BMC genomics · 2024Article
- Characterization of aFrontiers in cellular and infection microbiology · 2024Article
- Role of hypothetical protein PA1-LRP in antibacterial activity of endolysin from a newFrontiers in microbiology · 2024Article
- Strategies to combat Gram-negative bacterial resistance to conventional antibacterial drugs: a review.Osong public health and research perspectives · 2023Article
- Characterization of the LysP2110-HolP2110 Lysis System inInternational journal of molecular sciences · 2023Article
- Characterization and comprehensive genome analysis of novel bacteriophage, vB_Kpn_ZCKp20p, with lytic and anti-biofilm potential against clinical multidrug-resistantFrontiers in cellular and infection microbiology · 2023Article
- Functional Analysis of the Endopeptidase and Holin FromFrontiers in microbiology · 2022Article
- Endolysin, a Promising Solution against Antimicrobial Resistance.Antibiotics (Basel, Switzerland) · 2021Review
- Article
- Phages and Enzybiotics in Food Biopreservation.Molecules (Basel, Switzerland) · 2021Review
- Bacteriophage-Derived Endolysins Applied as Potent Biocontrol Agents to Enhance Food Safety.Microorganisms · 2020Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Most double-stranded (ds) DNA phages utilize holin proteins to secrete endolysin for host peptidoglycan lysis. In contrast, several holin-independent endolysins with secretion sequences or signal-arrest-release (SAR) sequences are secreted via the Sec pathway. In this study, we characterized a novel lysis protein (M4Lys) encoded by the dsDNA phage BSPM4, whose lysis function is not dependent on either holin or the Sec pathway in vitro. In silico analysis of M4Lys revealed that it contains a putative virion protein domain and an unusual C-terminal transmembrane domain (TMD). Turbidity reduction assays and liquid chromatography-mass spectrometry using purified peptidoglycan showed that the virion protein domain of M4Lys has peptidoglycan lysis activity. In vitro overproduction of M4Lys in
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.