ArticleCells2020
Highly Sensitive and Multiplexed In-Situ Protein Profiling with Cleavable Fluorescent Streptavidin.
Article in Cells, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 13 citations in OpenAlex.
- Emerging technologies in the spatial proteomics landscape.Analytical and bioanalytical chemistry · 2026Review
- Highly Sensitive Spatial Proteomics with Multicolor Cleavable Fluorescent Tyramide.Chemical & biomedical imaging · 2025Article
- Single-cell spatial proteomics.Histology and histopathology · 2025Review
- Ultrasensitive and Multiplexed Protein Imaging with Clickable and Cleavable Fluorophores.Analytical chemistry · 2024Article
- Ultrasensitive and multiplexed protein imaging with clickable and cleavable fluorophores.bioRxiv : the preprint server for biology · 2023Article
- Multiplexed Single-Cell in Situ Protein Profiling.ACS measurement science au · 2022Review
- Ultrasensitive and Multiplexed Protein Imaging with Cleavable Fluorescent Tyramide and Antibody Stripping.International journal of molecular sciences · 2021Article
- Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide.Cells · 2021Article
- Multiplexed In Situ Protein Profiling with High-Performance Cleavable Fluorescent Tyramide.Molecules (Basel, Switzerland) · 2021Article
- Single-cell proteomic analysis.WIREs mechanisms of disease · 2021Review
- Single-Cell Analysis.Cells · 2020Article
- Highly Sensitive and Multiplexed Protein Imaging With Cleavable Fluorescent Tyramide Reveals Human Neuronal Heterogeneity.Frontiers in cell and developmental biology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The ability to perform highly sensitive and multiplexed in-situ protein analysis is crucial to advance our understanding of normal physiology and disease pathogenesis. To achieve this goal, we here develop an approach using cleavable biotin-conjugated antibodies and cleavable fluorescent streptavidin (CFS). In this approach, protein targets are first recognized by the cleavable biotin-labeled antibodies. Subsequently, CFS is applied to stain the protein targets. Though layer-by-layer signal amplification using cleavable biotin-conjugated orthogonal antibodies and CSF, the protein detection sensitivity can be enhanced at least 10-fold, compared with the current in-situ proteomics methods. After imaging, the fluorophore and the biotin unbound to streptavidin are removed by chemical cleavage. The leftover streptavidin is blocked by biotin. Upon reiterative analysis cycles, a large number of different proteins with a wide range of expression levels can be profiled in individual cells at the optical resolution. Applying this approach, we have demonstrated that multiple proteins are unambiguously detected in the same set of cells, regardless of the protein analysis order. We have also shown that this method can be successfully applied to quantify proteins in formalin-fixed paraffin-embedded (FFPE) tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.