ArticlePloS one2020
Deletion of MHY1 abolishes hyphae formation in Yarrowia lipolytica without negative effects on stress tolerance.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Yarrowia lipolytica and Komagataella phaffii (Pichia pastoris) as Hosts for Heterologous Expression of Lipases.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Adaptive Evolution Identifies MHY1-Mediated Transcriptional Reprogramming for Polyphenol Tolerance in Yarrowia lipolytica.Biotechnology journal · 2026Article
- Phenotyping transcription factors-related genotypes in Y. lipolytica across a range of industrially relevant process parameters and chemical stress conditions.Microbial cell factories · 2026Article
- High Cell Density Fermentation ofInternational journal of molecular sciences · 2026Article
- Scarless Genetic Engineering ofJournal of microbiology and biotechnology · 2025Article
- New Yarrowia lipolytica chassis strains for industrial enzyme production.Microbial cell factories · 2025Article
- Reprogramming yeast metabolism for customized starch-rich micro-grain through low-carbon microbial manufacturing.Nature communications · 2025Article
- Global transcription machinery engineering in Yarrowia lipolytica.FEMS yeast research · 2025Review
- Engineering Yarrowia lipolytica for the production of β-carotene by carbon and redox rebalancing.Journal of biological engineering · 2025Article
- An Interplay between Transcription Factors and Recombinant Protein Synthesis inInternational journal of molecular sciences · 2024Article
- ReprogrammingScience advances · 2024Article
- 'Mother(Nature) knows best' - hijacking nature-designed transcriptional programs for enhancing stress resistance and protein production in Yarrowia lipolytica; presentation of YaliFunTome database.Microbial cell factories · 2024Article
- Cinnamic acid and p-coumaric acid are metabolized to 4-hydroxybenzoic acid by Yarrowia lipolytica.AMB Express · 2023Article
- Abolishing storage lipids induces protein misfolding and stress responses in Yarrowia lipolytica.Journal of industrial microbiology & biotechnology · 2023Article
- Phosphate limitation as crucial factor to enhance yeast lipid production from short-chain fatty acids.Microbial biotechnology · 2023Article
- Production of Rhizopus oryzae lipase using optimized Yarrowia lipolytica expression system.FEMS yeast research · 2023Article
- Enhancing the thermotolerance and erythritol production ofFrontiers in bioengineering and biotechnology · 2023Article
- Article
- Genome-wide CRISPR-Cas9 screen reveals a persistent null-hyphal phenotype that maintains high carotenoid production in Yarrowia lipolytica.Biotechnology and bioengineering · 2022Article
- A unique, newly discovered four-member protein family involved in extracellular fatty acid binding in Yarrowia lipolytica.Microbial cell factories · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is a need for development of sustainable production processes for production of fats/oils and lipid derived chemicals. The dimorphic oleaginous yeast Yarrowia lipolytica is a promising organism for conversion of biomass hydrolysate to lipids, but in many such processes hyphae formation will be problematic. We have therefore constructed and compared the performance of strains carrying deletions in several published gene targets suggested to abolish hyphae formation (MHY1, HOY1 and CLA4). The MHY1-deletion was the only of the tested strains which did not exhibit hyphae formation under any of the conditions tested. The MHY1-deletion also had a weak positive effect on lipid accumulation without affecting the total fatty acid composition, irrespective of the nitrogen source used. MHY1 has been suggested to constitute a functional homolog of the stress responsive transcription factors MSN2/4 in Saccharomyces cerevisiae, the deletion of which are highly stress sensitive. However, the deletion of MHY1 displayed only minor difference on survival of a range of acute or long term stress and starvation conditions. We conclude that the deletion of MHY1 in Y.lipolytica is a reliable way of abolishing hyphae formation with few detectable negative side effects regarding growth, stress tolerance and lipid accumulation and composition.
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