ArticleAnnals of clinical and translational neurology2020
Reduced neurosteroid potentiation of GABA
Article in Annals of clinical and translational neurology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- Cys-loop receptors on cannabinoids: All high?Frontiers in physiology · 2022Pooled it
- Molecular mechanisms of neurosteroids in temporal lobe epilepsy: current insights and therapeutic perspectives.Molecular biology reports · 2025Review
- Status epilepticus: Updates on mechanisms and treatments.Epilepsia open · 2025Review
- Inhibitory and excitatory synaptic neuroadaptations in the diazepam tolerant brain.Neurobiology of disease · 2023Article
- Virally-induced expression of GABAProgress in neurobiology · 2022Article
- Anti-epileptic Kunitz-like peptides discovered in the branching coral Acropora digitifera through transcriptomic analysis.Archives of toxicology · 2022Article
- Neuronal excitatory-to-inhibitory balance is altered in cerebral organoid models of genetic neurological diseases.Molecular brain · 2021Article
- Progesterone modulates neuronal excitability bidirectionally.Neuroscience letters · 2021Review
- Increased expression of GABABrain communications · 2021Article
- Neurosteroids and Focal Epileptic Disorders.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveNeurosteroids regulate neuronal excitability by potentiating γ-aminobutyric acid type-A receptors (GABARs). In animal models of temporal lobe epilepsy, the neurosteroid sensitivity of GABARs is diminished and GABAR subunit composition is altered. We tested whether similar changes occur in patients with epilepsy and if depolarization-induced increases in neuronal activity can replicate this effect.
methodsWe determined GABAR α4 subunit expression in cortical tissue resected from pediatric epilepsy patients. Modulation of human GABARs by allopregnanolone and Ro15-4513 was measured in Xenopus oocytes using whole-cell patch clamp. To extend the findings obtained using tissue from epilepsy patients, we evaluated GABAR expression and modulation by allopregnanolone and Ro15-4513 in cultured rat hippocampal neurons exposed to high extracellular potassium (HK) to increase neuronal activity.
resultsExpression of α4 subunits was increased in pediatric cortical epilepsy specimens encompassing multiple pathologies. The potentiation of GABA-evoked currents by the neurosteroid allopregnanolone was decreased in Xenopus oocytes expressing GABARs isolated from epilepsy patients. Furthermore, receptors isolated from epilepsy but not control tissue were sensitive to potentiation by Ro15-4513, indicating higher expression of α
interpretationThese findings suggest that seizure activity-induced upregulation of α
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