ReviewMolecular biology reports2020
Gene transfer to plants by electroporation: methods and applications.
Review in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 75 citations in OpenAlex.
- Plant Biofactories for Vaccination: Progress in Edible Vaccine Technologies as a Sustainable Approach to Global Immunization.Plant foods for human nutrition (Dordrecht, Netherlands) · 2026Review
- Genetically Modified Plants in Agriculture.Biology · 2026Review
- Tiny but powerful: protoplast isolation inFrontiers in plant science · 2026Article
- Advances in Genetic Engineering Techniques for Improved Forest Trees: Applications in Biomass, Stress Resilience and Carbon Sequestration.International journal of molecular sciences · 2025Review
- Electroporation-Mediated Gene Delivery in Pyropia yezoensis (Rhodophyta) Without Cell Wall Removal.Marine biotechnology (New York, N.Y.) · 2025Article
- Application of CRISPR-Cas9 in microbial cell factories.Biotechnology letters · 2025Review
- Development of Protoplast-Based Gene Editing System for Areca Palm.Plants (Basel, Switzerland) · 2025Article
- The role of nanoparticles in transforming plant genetic engineering: advancements, challenges and future prospects.Functional & integrative genomics · 2025Review
- DNA delivery into plant tissues using carbon dots made from citric acid and β-alanine.Frontiers in chemistry · 2025Article
- Advancements in plant transformation: from traditional methods to cutting-edge techniques and emerging model species.Plant cell reports · 2024Review
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- Direct protein delivery into intact Arabidopsis cells for genome engineering.Scientific reports · 2024Article
- CRISPR/Cas genome editing in plants: mechanisms, applications, and overcoming bottlenecks.Functional & integrative genomics · 2024Review
- Recent Advances in Tomato Gene Editing.International journal of molecular sciences · 2024Review
- Transport of Nanoparticles into Plants and Their Detection Methods.Nanomaterials (Basel, Switzerland) · 2024Review
- Development of virus-induced genome editing methods in Solanaceous crops.Horticulture research · 2024Article
- Isolation, Purification, and Application of Protoplasts and Transient Expression Systems in Plants.International journal of molecular sciences · 2023Review
- Application of Nanotechnology in Plant Genetic Engineering.International journal of molecular sciences · 2023Review
- Technological Development and Application of Plant Genetic Transformation.International journal of molecular sciences · 2023Review
- Identification of Proteins Involved in Cell Membrane Permeabilization by Nanosecond Electric Pulses (nsEP).International journal of molecular sciences · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Developing gene transfer technologies enables the genetic manipulation of the living organisms more efficiently. The methods used for gene transfer fall into two main categories; natural and artificial transformation. The natural methods include the conjugation, transposition, bacterial transformation as well as phage and retroviral transductions, contain the physical methods whereas the artificial methods can physically alter and transfer genes from one to another organisms' cell using, for instance, biolistic transformation, micro- and macroinjection, and protoplast fusion etc. The artificial gene transformation can also be conducted through chemical methods which include calcium phosphate-mediated, polyethylene glycol-mediated, DEAE-Dextran, and liposome-mediated transfers. Electrical methods are also artificial ways to transfer genes that can be done by electroporation and electrofusion. Comparatively, among all the above-mentioned methods, electroporation is being widely used owing to its high efficiency and broader applicability. Electroporation is an electrical transformation method by which transient electropores are produced in the cell membranes. Based on the applications, process can be either reversible where electropores in membrane are resealable and cells preserve the vitality or irreversible where membrane is not able to reseal, and cell eventually dies. This problem can be minimized by developing numerical models to iteratively optimize the field homogeneity considering the cell size, shape, number, and electrode positions supplemented by real-time measurements. In modern biotechnology, numerical methods have been used in electrotransformation, electroporation-based inactivation, electroextraction, and electroporative biomass drying. Moreover, current applications of electroporation also point to some other uncovered potentials for various exploitations in future.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.