ArticleProceedings of the National Academy of Sciences of the United States of America2020
Computational design of probes to detect bacterial genomes by multivalent binding.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.
- Effect of Intensity and Duration of Exercise on Gut Microbiota in Humans: A Systematic Review.International journal of environmental research and public health · 2022Pooled it
- Fluorescent carbon dot-based biosensor for the rapid and sensitive detection of Escherichia coli DNA.Scientific reports · 2026Article
- Computational Framework for High Copy-Number Probe Selection and Cross-Binding Reduction.Analytical science advances · 2025Article
- Reduced graphene oxide-based absorbance biosensors for detecting Escherichia coli DNA.Scientific reports · 2025Article
- Hopping and crawling DNA-coated colloids.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Weak-cooperative binding of a long single-stranded DNA chain on a surface.Nucleic acids research · 2024Article
- Heteromultivalency enables enhanced detection of nucleic acid mutations.Nature chemistry · 2024Article
- Whole-genome detection using multivalent DNA-coated colloids.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Determinants of Superselectivity─Practical Concepts for Application in Biology and Medicine.Accounts of chemical research · 2023Article
- Simple Complexity: Incorporating Bioinspired Delivery Machinery within Self-Assembled Peptide Biogels.Gels (Basel, Switzerland) · 2023Review
- Dynamic Light Scattering Based Microrheology of End-Functionalised Triblock Copolymer Solutions.Polymers · 2023Article
- Amplification Free Detection of SARS-CoV-2 Using Multi-Valent Binding.ACS sensors · 2022Article
- Significance of Receptor Mobility in Multivalent Binding on Lipid Membranes.Angewandte Chemie (International ed. in English) · 2022Review
- Novel Biorecognition Elements against Pathogens in the Design of State-of-the-Art Diagnostics.Biosensors · 2021Review
- Binding Revisited-Avidity in Cellular Function and Signaling.Frontiers in molecular biosciences · 2020Review
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid methods for diagnosis of bacterial infections are urgently needed to reduce inappropriate use of antibiotics, which contributes to antimicrobial resistance. In many rapid diagnostic methods, DNA oligonucleotide probes, attached to a surface, bind to specific nucleotide sequences in the DNA of a target pathogen. Typically, each probe binds to a single target sequence; i.e., target-probe binding is monovalent. Here we show using computer simulations that the detection sensitivity and specificity can be improved by designing probes that bind multivalently to the entire length of the pathogen genomic DNA, such that a given probe binds to multiple sites along the target DNA. Our results suggest that multivalent targeting of long pieces of genomic DNA can allow highly sensitive and selective binding of the target DNA, even if competing DNA in the sample also contains binding sites for the same probe sequences. Our results are robust to mild fragmentation of the bacterial genome. Our conclusions may also be relevant for DNA detection in other fields, such as disease diagnostics more broadly, environmental management, and food safety.
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