ArticleACS nano2020
Subcellular Mass Spectrometry Imaging and Absolute Quantitative Analysis across Organelles.
Article in ACS nano, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 77 citations in OpenAlex.
- Review
- Mass spectrometry imaging for unearthing and validating quality markers in traditional Chinese medicines.Chinese herbal medicines · 2025Review
- Deciphering breast cancer dynamics: insights from single-cell and spatial profiling in the multi-omics era.Biomarker research · 2024Review
- Tool to Resolve Distortions in Elemental and Isotopic Imaging.Journal of the American Chemical Society · 2024Article
- Gel-assisted mass spectrometry imaging enables sub-micrometer spatial lipidomics.Nature communications · 2024Article
- Single Cell mass spectrometry: Towards quantification of small molecules in individual cells.Trends in analytical chemistry : TRAC · 2024Article
- Mass spectrometry imaging of metals in tissues and cells: Methods and biological applications.Biochimica et biophysica acta. General subjects · 2024Review
- Mass spectrometry imaging as a promising analytical technique for herbal medicines: an updated review.Frontiers in pharmacology · 2024Review
- Spatial pharmacology using mass spectrometry imaging.Trends in pharmacological sciences · 2024Review
- Bidirectional interplay between metabolism and epigenetics in hematopoietic stem cells and leukemia.The EMBO journal · 2023Review
- Next Generation of Mass Spectrometry Imaging: from Micrometer to Subcellular Resolution.Chemical & biomedical imaging · 2023Review
- Exploiting Mass Spectrometry to Unlock the Mechanism of Nanoparticle-Induced Inflammasome Activation.ACS nano · 2023Article
- Subcellular quantitative imaging of metabolites at the organelle level.Nature metabolism · 2023Article
- The evolving role of investigative toxicology in the pharmaceutical industry.Nature reviews. Drug discovery · 2023Review
- Recent developments in ionization techniques for single-cell mass spectrometry.Frontiers in chemistry · 2023Review
- Mass spectrometry imaging: new eyes on natural products for drug research and development.Acta pharmacologica Sinica · 2022Review
- Principles and functions of metabolic compartmentalization.Nature metabolism · 2022Review
- Evaluation of 6 MALDI-Matrices for 10 μm Lipid Imaging and On-Tissue MSn with AP-MALDI-Orbitrap.Journal of the American Society for Mass Spectrometry · 2022Article
- Perspective on fluorescence cell imaging with ionophore-based ion-selective nano-optodes.Biomicrofluidics · 2022Article
- Simultaneous Counting of Molecules in the Halo and Dense-Core of Nanovesicles by Regulating Dynamics of Vesicle Opening.Angewandte Chemie (International ed. in English) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mass spectrometry imaging is a field that promises to become a mainstream bioanalysis technology by allowing the combination of single-cell imaging and subcellular quantitative analysis. The frontier of single-cell imaging has advanced to the point where it is now possible to compare the chemical contents of individual organelles in terms of raw or normalized ion signal. However, to realize the full potential of this technology, it is necessary to move beyond this concept of relative quantification. Here we present a nanoSIMS imaging method that directly measures the absolute concentration of an organelle-associated, isotopically labeled, pro-drug directly from a mass spectrometry image. This is validated with a recently developed nanoelectrochemistry method for single organelles. We establish a limit of detection based on the number of isotopic labels used and the volume of the organelle of interest, also offering this calculation as a web application. This approach allows subcellular quantification of drugs and metabolites, an overarching and previously unmet goal in cell science and pharmaceutical development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.