ArticleThe Plant journal : for cell and molecular biology2020
Arabidopsis Raf-like kinases act as positive regulators of subclass III SnRK2 in osmostress signaling.
Article in The Plant journal : for cell and molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers, 1 of them a synthesis that pooled it.
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55 citing papers in PubMed, 1 synthesis or guideline pooled it, 125 citations in OpenAlex.
- Dry side of the core: a meta-analysis addressing the original nature of the ABA signalosome at the onset of seed imbibition.Frontiers in plant science · 2023Pooled it
- Aspects of plant intelligence (ii).Plant signaling & behavior · 2026Review
- A RAF-SnRK2-PP2C functional module regulates cell survival in the chlorophyte Chlamydomonas reinhardtii.The New phytologist · 2026Article
- The MPK6-WRKY49 module links MAPK signaling to ABA biosynthesis and drought tolerance in Arabidopsis.The Plant journal : for cell and molecular biology · 2026Article
- Protein Post-Translational Modifications in Plant Abiotic Stress Responses.Plants (Basel, Switzerland) · 2025Review
- MAP4K1 and MAP4K2 regulate ABA-induced and CaScience advances · 2025Article
- Maintaining basal B-RAF kinase activity for abscisic acid signaling via reciprocal phosphoregulation of a single serine residue.Journal of integrative plant biology · 2025Article
- One arrow-two targets: Dual role of ETHYLENE INSENSITIVE2-like protein in osmostress and submergence tolerance.Plant physiology · 2025Article
- Identification of a novel stomatal opening chemical, PP242, that inhibits early abscisic acid signal transduction in guard cells.Plant & cell physiology · 2025Article
- ETHYLENE INSENSITIVE2-like protein mediates submergence and drought responses in Physcomitrium patens.Plant physiology · 2025Article
- Transcriptional gene network involved in drought stress response: application for crop breeding in the context of climate change.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- SnRK2s: Kinases or Substrates?Plants (Basel, Switzerland) · 2025Review
- Comparative physiological and co-expression network analysis reveals potential hub genes and adaptive mechanisms responsive to NaCl stress in peanut (Arachis hypogaea L.).BMC plant biology · 2025Article
- DOG1 controls dormancy independently of ABA core signaling kinases regulation by preventing AFP dephosphorylation through AHG1.Science advances · 2025Article
- Hyperosmolarity-induced suppression of group B1 Raf-like protein kinases modulates drought-growth trade-off inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39 regulate immune homeostasis and stomatal opening in Arabidopsis thaliana.The New phytologist · 2024Article
- Regulatory networks in plant responses to drought and cold stress.Plant physiology · 2024Review
- ABI5-FLZ13 module transcriptionally represses growth-related genes to delay seed germination in response to ABA.Plant communications · 2023Article
- Silencing theInternational journal of molecular sciences · 2023Article
- The osmotic stress-activated receptor-like kinase DPY1 mediates SnRK2 kinase activation and drought tolerance in Setaria.The Plant cell · 2023Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Given their sessile nature, land plants must use various mechanisms to manage dehydration under water-deficit conditions. Osmostress-induced activation of the SNF1-related protein kinase 2 (SnRK2) family elicits physiological responses such as stomatal closure to protect plants during drought conditions. With the plant hormone ABA receptors [PYR (pyrabactin resistance)/PYL (pyrabactin resistance-like)/RCAR (regulatory component of ABA receptors) proteins] and group A protein phosphatases, subclass III SnRK2 also constitutes a core signaling module for ABA, and osmostress triggers ABA accumulation. How SnRK2 is activated through ABA has been clarified, although its activation through osmostress remains unclear. Here, we show that Arabidopsis ABA and abiotic stress-responsive Raf-like kinases (AtARKs) of the B3 clade of the mitogen-activated kinase kinase kinase (MAPKKK) family are crucial in SnRK2-mediated osmostress responses. Disruption of AtARKs in Arabidopsis results in increased water loss from detached leaves because of impaired stomatal closure in response to osmostress. Our findings obtained in vitro and in planta have shown that AtARKs interact physically with SRK2E, a core factor for stomatal closure in response to drought. Furthermore, we show that AtARK phosphorylates S171 and S175 in the activation loop of SRK2E in vitro and that Atark mutants have defects in osmostress-induced subclass III SnRK2 activity. Our findings identify a specific type of B3-MAPKKKs as upstream kinases of subclass III SnRK2 in Arabidopsis. Taken together with earlier reports that ARK is an upstream kinase of SnRK2 in moss, an existing member of a basal land plant lineage, we propose that ARK/SnRK2 module is evolutionarily conserved across 400 million years of land plant evolution for conferring protection against drought.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.